Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

12.3K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.3K
Sanger Sequencing01:57

Sanger Sequencing

777.0K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
777.0K
Next-generation Sequencing03:00

Next-generation Sequencing

100.1K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
100.1K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

13.4K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
13.4K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

804
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
804
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

7.5K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
7.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

4-N-furfurylcytosine, a novel cytosine-derived modulator of mitochondrial and redox homeostasis to counteract aging: Insights from cellular and mouse models.

Free radical biology & medicine·2026
Same author

Beyond the complex: inosine drives the antiviral and epigenetic effects of inosine pranobex.

Journal of applied genetics·2026
Same author

The Year 2026 in Biology: A Jubilee Year

Postepy biochemii·2026
Same author

Labeling of Amine-Modified Material Surfaces with PFB-Fluorescein.

Nanotechnology, science and applications·2026
Same author

The Anticancer Drug Mitoxantrone Triggers the Formation of Ribosome-enriched Stress Granules Independently of the Classical Translational Control Pathways.

Journal of molecular biology·2026
Same author

Molecular background behind lateral elbow pain reduction with Leukocyte-Rich and Leukocyte-Poor Platelet-Rich Plasma-randomized control trial and single-cell platelet analysis.

International orthopaedics·2026
Same journal

Elemental changes in tooth hard tissues during demineralization and fluoride-induced remineralization: an SEM-EDX study.

Acta biochimica Polonica·2026
Same journal

Precise genome modification of agrobacterium based on one-step homologous recombination via endogenous transfer DNA.

Acta biochimica Polonica·2026
Same journal

Recommendations of the Polish Zebrafish Society on the use of the zebrafish (<i>Danio rerio</i>) model in biomedical research.

Acta biochimica Polonica·2026
Same journal

Pilot analysis of protein profile alterations in plasma and aortic tissue of spontaneously hypertensive rats.

Acta biochimica Polonica·2026
Same journal

Skin examination in extreme conditions.

Acta biochimica Polonica·2026
Same journal

Genetic architecture of obesity and advances in precision pharmacotherapy: a comprehensive review.

Acta biochimica Polonica·2026
See all related articles

Related Experiment Video

Updated: Mar 11, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.6K

RNA dactyloscopy.

Marta Magdalena Gabryelska1, Eliza Wyszko2, Jan Barciszewski2

  • 1Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland; Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom.

Acta Biochimica Polonica
|December 2, 2016
PubMed
Summary
This summary is machine-generated.

Predicting RNA biological activity is challenging. RNA dactyloscopy, a novel in silico method, aids in designing efficient hammerhead ribozymes for intracellular applications.

More Related Videos

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

14.9K
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.5K

Related Experiment Videos

Last Updated: Mar 11, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.6K
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

14.9K
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.5K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Computational Biology

Background:

  • Extensive data exists on RNA structure and dynamics.
  • Predicting RNA biological activity within cellular environments remains a significant challenge.

Purpose of the Study:

  • To present a novel computational method for RNA design.
  • To enhance the intracellular efficiency of hammerhead ribozymes.

Main Methods:

  • In silico RNA fingerprinting (RNA dactyloscopy).
  • Comparison with published experimental data.

Main Results:

  • RNA dactyloscopy proves to be a reliable tool for assessing catalytic properties.
  • The method facilitates the design of hammerhead ribozymes with high intracellular efficiency.

Conclusions:

  • RNA dactyloscopy is effective for RNA modeling and design.
  • This approach aids in understanding and predicting RNA biological activity.