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Resonant nanopillars arrays for label-free biosensing
Optics Letters
|December 2, 2016
Summary
Resonant nanopillar (R-NP) arrays show promise as label-free, multiplexed optical biosensors. This study demonstrates their capability for detecting biomolecular interactions on a chip.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Optical Sensing
Background:
- Previous work established resonant nanopillars (R-NP) as biochemical transducers.
- R-NP arrays, fabricated from Si3N4/SiO2 Bragg reflectors with a SiO2 cavity, offer a novel platform for biosensing.
Purpose of the Study:
- To demonstrate the capability and suitability of R-NP arrays on a chip as label-free optical multiplexed biosensors.
- To validate the performance of R-NP arrays in detecting specific biomolecular interactions.
Main Methods:
- Biofunctionalization of R-NP surfaces with IgG antibodies via silanization using APTMS.
- Integration of R-NP into eight independent sensing arrays on a quartz chip.
- Vertical and independent optical monitoring of each sensing array using an optical fiber bundle.
Main Results:
- Successful immobilization of antibodies and subsequent detection of anti-IgG binding.
- Demonstration of label-free detection with increasing concentrations of analytes.
- Achieved promising performance and yield for the multiplexed biosensor system.
Conclusions:
- R-NP arrays are suitable for label-free, multiplexed optical biosensing applications.
- The developed chip-based biosensor shows potential for sensitive and efficient biomolecular detection.
- This technology offers a novel platform for advanced biosensing.
