Cytogenetic analysis of two C-1300 murine neuroblastoma cell lines expressing discordant malignant behavior

Cytogenetic analysis of two murine neuroblastoma cell lines that show different malignant expression revealed consistent differences in the chromosomal composition between the two lines. The MNB-T1 (high-malignancy) cell line showed increased modal chromosome number, presence of homogeneously staining regions (HSRs), double minutes (dmin) and evidence of secondary chromosomal events when compared with the MNB-T2 (low-malignancy) cell line, which did not express these cytogenetic characteristics. Several of the cytogenetic events that have occurred in these murine cell lines, such as HSRs, dmin, and chromosomal deletions, have also been reported in human neuroblastoma cell lines. When comparisons are made for the involvement of chromosomal regions known to be involved in human neuroblastoma, similar tumor specific regions seem to be involved in the homologous mouse chromosomes.