[Determination of paracetamol in the blood using high-performance liquid chromatography]

The paper reports the determination of paracetamol in the serum by means of high-performance liquid chromatography. Prior to analysis, the sample is purified by deproteination with perchloric acid. The analysis is carried out isocratically on the reverse phase (SEPARON SIX C18, 5 microns, 150 x x 3.7 mm). The mobile phase consists of 40% methanol in 0.4% phosphoric acid. The detection is performed at 254 nm. The calibration curve is linear in the region 6.62-331 mumol.l-1 (1.0-50.0 micrograms.ml-1) with 95% reproducibility. The sensitivity of detection is 1.3 mumol.l-1 (0.2 microgram.ml-1). The suitability of the method was checked by processing 300 serum samples.