[Isolation of monoclonal antibodies from mouse ascitic fluid using chromatography on "blue" DEAE cellulose]

A method is described of the isolation of monoclonal antibodies form mouse ascites fluids by chromatography on beaded DEAE cellulose with covalently bound Reactive Blue-2 ("Blue" DEAE cellulose). The method yields antibodies of 80-90% purity, free from protease activity.