Correction of Negative Effect of Antenatal Hypoxia on Liver Tissue Homeostasis in Newborn Albino Rats with Opioid
O G Pinaeva1, E N Sazonova2,3, O A Lebed'ko2,3
1Far Eastern State Medical University, Ministry of Health of Russian Federation, Khabarovsk, Russia. pinaeva_og@mail.ru.
Insights
Opioid peptides like Dalargin can correct liver damage from prenatal hypoxia in rat pups. Both tested peptides reduced oxidative stress and improved antioxidant defenses in the liver tissue.
Area of Science:
- Biomedical Science
- Pharmacology
- Toxicology
Background:
- Antenatal hypoxia can negatively impact liver tissue homeostasis in newborns.
- Opioid peptides are being investigated for their potential therapeutic effects.
Purpose of the Study:
- To investigate the efficacy of opioid peptides in correcting antenatal hypoxia-induced liver damage in neonatal rats.
- To evaluate the impact of Dalargin and a Leu-enkephalin analogue on liver tissue homeostasis.
Main Methods:
- Neonatal albino rats exposed to antenatal hypoxia were treated with opioid peptides (Dalargin, Leu-enkephalin analogue) from postnatal days 2-6.
- Assessment included gravimetric indicators, hepatocyte proliferative activity, nucleolar apparatus function, and markers of oxidative stress and antioxidant defense.
Main Results:
- Dalargin normalized gravimetric parameters, proliferative activity, and hepatocyte nucleolar apparatus function.
- The Leu-enkephalin analogue increased hepatocyte proliferation but did not normalize other parameters.
- Both peptides significantly reduced free radical oxidation and improved antioxidant defense and peroxidation resistance.
Conclusions:
- The mixed μ/δ-opioid receptor agonist Dalargin effectively corrects liver tissue homeostasis disruptions caused by antenatal hypoxia.
- Opioid peptides demonstrate potential in mitigating oxidative stress and enhancing antioxidant capacity in the context of prenatal hypoxia.
- Further research into opioid peptide therapy for neonatal hypoxic injury is warranted.
Abstract:
We studied the possibility of correction of the negative effects of antenatal hypoxia on the liver tissue homeostasis in 7-day-old albino rats by administration of opioid peptides in a dose of 100 μg/kg on postnatal days 2-6. Administration of mixed μ/δ-opioid receptor agonist Dalargin neutralized deviations of gravimetric indicators, parameters of proliferative activity, and activity of the nucleolar apparatus of hepatocytes. Administration of the non-opiate Leu-enkephalin analogue did not normalize gravimetric parameters and nucleolar apparatus parameters, however, it significantly increased the pool of proliferating hepatocytes. Both peptides significantly reduced the intensity of free radical oxidation, improved antioxidant antiradical defense and resistance to peroxidation in the liver tissue of animals subjected to antenatal hypoxia.
