Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Karyotyping01:17

Karyotyping

69.5K
Overview
69.5K
Cancer02:18

Cancer

55.3K
Cancers arise due to mutations in genes involved in the regulation of cell division, which leads to unrestricted cell proliferation. Modern science and medicine have made great strides in the understanding and treatment of cancer, including eradicating cancer in some patients. However, there is still no cure for cancer. This is largely due to the fact that cancer is a large group of many diseases.
55.3K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

11.7K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
11.7K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

10.0K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
10.0K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

15.2K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
15.2K
What is Cancer?02:12

What is Cancer?

15.3K
Cells and tissues must meticulously coordinate their activities for the normal functioning of the human body. Therefore, they exhibit socially responsible behavior - resting, growing, dividing, differentiating, or dying - for the organism’s benefit. Cancer arises when cells divide uncontrollably and invade other tissues or organs.
Although people have known about cancer for centuries, it was only in 1761 that Giovanni Morgagni of Padua performed a detailed autopsy of...
15.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A multi-modal molecular characterization of the Philadelphia translocation featuring long read sequencing.

Gene·2025
Same author

Chromosome Bandings and Recognition.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Application of droplet digital PCR in minimal residual disease monitoring of rare fusion transcripts and mutations in haematological malignancies.

Scientific reports·2024
Same author

Deep genomic characterization highlights complexities and prognostic markers of pediatric acute myeloid leukemia.

Communications biology·2023
Same author

A novel NUP98-JADE2 fusion in a patient with acute myeloid leukemia resembling acute promyelocytic leukemia.

Blood advances·2021
Same author

Rapid detection of chromosomal translocation and precise breakpoint characterization in acute myeloid leukemia by nanopore long-read sequencing.

Cancer genetics·2019

Related Experiment Video

Updated: Mar 11, 2026

Chromosome Preparation From Cultured Cells
07:42

Chromosome Preparation From Cultured Cells

Published on: January 28, 2014

84.2K

Cancer Cytogenetics: An Introduction.

Thomas S K Wan1

  • 1Haematology Division, Department of Anatomical & Cellular Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong. thomaswan@cuhk.edu.hk.

Methods in Molecular Biology (Clifton, N.J.)
|December 3, 2016
PubMed
Summary

Cancer cytogenetics has evolved significantly, improving cancer diagnosis and treatment. Advanced techniques like fluorescence in situ hybridization (FISH) and DNA microarrays enhance the detection of chromosomal alterations, guiding personalized medicine.

Keywords:
Cancer cytogeneticsFISHKaryotypingMolecular cytogeneticsReview

More Related Videos

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
09:49

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

Published on: July 5, 2019

10.1K
Spectral Karyotyping to Study Chromosome Abnormalities in Humans and Mice with Polycystic Kidney Disease
12:47

Spectral Karyotyping to Study Chromosome Abnormalities in Humans and Mice with Polycystic Kidney Disease

Published on: February 3, 2012

39.4K

Related Experiment Videos

Last Updated: Mar 11, 2026

Chromosome Preparation From Cultured Cells
07:42

Chromosome Preparation From Cultured Cells

Published on: January 28, 2014

84.2K
Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
09:49

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

Published on: July 5, 2019

10.1K
Spectral Karyotyping to Study Chromosome Abnormalities in Humans and Mice with Polycystic Kidney Disease
12:47

Spectral Karyotyping to Study Chromosome Abnormalities in Humans and Mice with Polycystic Kidney Disease

Published on: February 3, 2012

39.4K

Area of Science:

  • Cytogenetics
  • Cancer Genomics
  • Molecular Biology

Background:

  • The Philadelphia chromosome, discovered in 1960, marked the first chromosomal abnormality linked to cancer, specifically chronic myeloid leukemia.
  • Over five decades, cancer cytogenetics has advanced significantly, improving the detection of chromosomal alterations in neoplasms.

Purpose of the Study:

  • To highlight the evolution and impact of cytogenetic techniques in cancer research and diagnostics.
  • To emphasize the role of advanced methods in understanding cancer progression and developing targeted therapies.

Main Methods:

  • Karyotyping for single-cell analysis to understand clonal evolution.
  • Advanced fluorescence in situ hybridization (FISH) for identifying chromosomal alterations beyond karyotyping.
  • DNA microarray technologies for high-resolution whole-genome analysis (cancer cytogenomics).

Main Results:

  • Cytogenetic analysis, especially single-cell studies, remains crucial for understanding cancer cell clonal evolution and disease progression.
  • FISH and DNA microarrays overcome limitations of traditional karyotyping, offering higher resolution and comprehensive genomic views.
  • These advancements provide critical information for improving cancer patient care and identifying therapeutic targets.

Conclusions:

  • Cancer cytogenetics has transformed cancer diagnostics and research, moving towards personalized medicine.
  • Technological innovations in cytogenetics are pivotal for identifying cancer-driving genes and enabling targeted therapies.
  • The field of cancer cytogenomics offers vast potential for future cancer research and treatment strategies.