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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

100.1K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

13.4K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Genome Annotation and Assembly03:36

Genome Annotation and Assembly

21.3K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Updated: Mar 11, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

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Next-Generation Sequencing.

Matthieu Le Gallo1, Fred Lozy1, Daphne W Bell2

  • 1Cancer Genetics and Comparative Genomics Branch, National Human Genome Research Institute, National Institutes of Health, 50 South Drive, Bethesda, MD, 20892, USA.

Advances in Experimental Medicine and Biology
|December 3, 2016
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing reveals four distinct molecular subgroups of endometrial cancer. This advancement aids in discovering new cancer genes driving serous and endometrioid tumor development.

Keywords:
CancerEndometrialExomeGeneticGenomicMutationNext-generation sequencingUterine

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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

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Nanopore DNA Sequencing for Metagenomic Soil Analysis
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Nanopore DNA Sequencing for Metagenomic Soil Analysis

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Related Experiment Videos

Last Updated: Mar 11, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
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Nanopore DNA Sequencing for Metagenomic Soil Analysis
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Area of Science:

  • Oncology
  • Genomics
  • Molecular Biology

Background:

  • Endometrial cancer is a common gynecological malignancy, often driven by genetic mutations.
  • Previous methods like Sanger sequencing limited comprehensive analysis of cancer genes.
  • Next-generation sequencing (NGS) offers a cost-effective and high-throughput approach to study tumor genomes.

Purpose of the Study:

  • To classify endometrial cancers into distinct molecular subgroups using NGS.
  • To identify novel cancer genes associated with endometrial carcinomas.
  • To provide an overview of the genomic features characterizing these subgroups.

Main Methods:

  • Whole-exome, transcriptome, and genome sequencing of endometrial tumors.
  • Analysis of somatic mutations to identify distinct molecular profiles.
  • Comparison of genomic features across different endometrial cancer subtypes.

Main Results:

  • NGS identified four distinct molecular subgroups within serous and endometrioid endometrial cancers.
  • Cataloged the mutational landscapes of endometrial tumor exomes, transcriptomes, and genomes.
  • Highlighted known and putative cancer genes driving tumorigenesis in these subgroups.

Conclusions:

  • Endometrial cancer classification can be refined through molecular subtyping.
  • NGS technologies are crucial for comprehensive cancer gene discovery.
  • Understanding these molecular subgroups may lead to targeted therapies for endometrial cancer.