Related Experiment Video

Updated: Mar 11, 2026

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
06:47

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

9.9K

microRNA-25 targets PKCζ and protects osteoblastic cells from dexamethasone via activating AMPK signaling

Jian-Bo Fan1,2, Wei Liu1, Xin-Hui Zhu1

  • 1Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, PR China.

Oncotarget
|December 3, 2016
PubMed

Insights

MicroRNA-25-5p protects osteoblasts from dexamethasone by downregulating protein kinase C ζ (PKCζ), activating AMP-activated protein kinase (AMPK), and reducing oxidative stress.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Osteoblasts are crucial for bone health.
  • Dexamethasone (Dex) can induce osteoblast damage.
  • AMP-activated protein kinase (AMPK) activation shows potential for osteoblast protection.

Purpose of the Study:

  • To investigate the role of microRNA-25-5p (miR-25-5p) in protecting osteoblasts from dexamethasone (Dex).
  • To explore the mechanism involving protein kinase C ζ (PKCζ) and AMPK activation.

Main Methods:

  • Utilized human osteoblastic cell lines (OB-6 and hFOB1.19).
  • Manipulated miR-25-5p levels via forced expression and antagomiR-25.
  • Assessed protein levels of PKCζ and AMPK activation.
  • Investigated effects on NADPH activity and oxidative stress.
  • Correlated findings with patient tissue samples.

Main Results:

  • miR-25-5p targets and downregulates PKCζ in osteoblasts.
  • miR-25-5p activation of AMPK protects osteoblasts from Dex-induced damage.
  • PKCζ downregulation and AMPK activation are essential for miR-25-5p's protective effects.
  • miR-25-5p increases NADPH activity and reduces oxidative stress, effects dependent on AMPK.
  • Increased miR-25-5p levels and decreased PKCζ were observed in patient necrotic femoral head tissues.

Conclusions:

  • miR-25-5p protects osteoblastic cells from dexamethasone.
  • This protection is mediated by targeting PKCζ, leading to AMPK activation.
  • The miR-25-5p/PKCζ/AMPK pathway represents a potential therapeutic target for dexamethasone-induced osteoblast injury.
Keywords:
AMP-activated protein kinase (AMPK)PKCζdexamethasonemicroRNA-25osteoblastic cells

More Related Videos

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation09:37

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Published on: March 15, 2018

14.9K
Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle10:05

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Published on: March 5, 2019

6.9K

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
6.1K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.5K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.0K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
9.0K