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Characterization of Aquatic Biofilms with Flow Cytometry

Characterization of Aquatic Biofilms with Flow Cytometry

Published on: June 6, 2018

Source tracking using microbial community fingerprints: Method comparison with hydrodynamic modelling.

D T McCarthy¹, D Jovanovic¹, A Lintern¹

¹Environmental and Public Health Microbiology Laboratory (EPHM Lab), Civil Engineering Department, Monash University, VIC, Australia.

|December 3, 2016

Summary

This summary is machine-generated.

Microbial source tracking (MST) using bacterial communities effectively identified water sources contributing to contamination in the Yarra River estuary. This method shows potential for managing urban waterway pollution.

Keywords:

Community profiling Faecal source tracking Microbial source tracking (MST)SourceTracker Tuflow Urban stormwater

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Area of Science:

Environmental microbiology
Estuarine science
Water quality management

Background:

Urban estuaries face contamination from various sources, posing public health risks.
Identifying primary water sources of contamination in catchments is crucial for risk mitigation.
Microbial source tracking (MST) is a tool for identifying contamination sources, but requires validation.

Purpose of the Study:

To identify major water sources of bacterial loadings in an urban estuary using MST.
To evaluate the effectiveness of MST by comparing its results with a 3D hydrodynamic model.
To assess the potential of microbial community MST for managing contaminated aquatic systems.

Main Methods:

Microbial source tracking (MST) using bacterial community fingerprints.
Analysis of water samples from the Yarra River estuary, Melbourne, Australia.
Comparison with a 3-dimensional hydrodynamic model simulating tracer transport and mixing.

Main Results:

Water sources contributing to bacterial communities varied temporally with hydrodynamic conditions.
MST-derived water source apportionment correlated with results from the hydrodynamic model.
MST using bacterial community fingerprints successfully identified primary microbial sources in the estuary.

Conclusions:

Microbial community MST is a viable tool for identifying water sources of microorganisms in estuarine environments.
The study validated MST by comparing it with an independent hydrodynamic model.
MST has potential for practical application in mitigating contamination in urban aquatic systems with further development.