Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Precipitation Titration: Endpoint Detection Methods

Precipitation Titration: Endpoint Detection Methods

In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
In the Volhard method, a standard excess of AgNO3 is first added to the...

Complexometric Titration: Overview

Complexometric Titration: Overview

Complexometric titration involves the formation of a complex by reacting a metal ion with one or more ligands. A visual indicator often detects the end point of a complexometric titration. It is added to the metal solution before the titration, forming a stable metal–indicator complex and imparting color to the solution. As the titration approaches the equivalence point, the excess of the added ligand displaces the indicator from the metal–indicator complex, releasing the free...

Effects of EDTA on End-Point Detection Methods

Effects of EDTA on End-Point Detection Methods

Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Determination of the new psychoactive substances MDMB-4en-PINACA, ADB-BUTINACA and some of their metabolites in blood and urine using DLLE-LC-MS/MS: application to real forensic case samples.

Forensic science international·2026

Same author

Zinc, Selenium, and Iodine Biofortification of Hard-Dough Biscuits Using Agronomically Biofortified Whole Wheat Flour: Mineral Retention, Physicochemical Properties, and Sensory Acceptability.

Journal of AOAC International·2026

Same author

Design, Synthesis, and Biological Evaluation of Methoxy-Substituted Chalcones and Gypsogenin-Based Hybrids as Anticancer and Antimicrobial Agents.

ACS omega·2026

Same author

Unveiling iron chemical speciation in low sample volumes by a spectroelectrochemical approach.

Talanta·2026

Same author

Oligosaccharides from Scorzonera yildirimlii and S. zorkunensis, and their potential antimicrobial, enzyme inhibition, cytotoxic effect, and in silico study.

Carbohydrate research·2026

Same author

Phytochemical analysis and antimicrobial, antioxidant, and cytotoxic activities of Scorzonera aucheriana DC (Asteraceae).

Fitoterapia·2026

Search research articles

Related Experiment Video

Updated: Mar 11, 2026

Author Spotlight: Assessing the Impact of Novel Iron Chelators on Cancer Cell Metabolism

Author Spotlight: Assessing the Impact of Novel Iron Chelators on Cancer Cell Metabolism

Published on: February 23, 2024

A Simple Spectrofluorimetric Method for Iron Determination with a Chalcone-Based Schiff Base.

Nurhayat Özbek¹, Hakan Alp¹, Gonca Çelik¹

¹Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.

Journal of Fluorescence

|December 4, 2016

Summary

A novel Schiff base effectively detects iron (III) in soil and food samples using a simple spectrofluorimetric method. This cost-effective approach offers accurate iron determination, crucial for food safety and environmental monitoring.

Keywords:

Chalcone Food Iron determination Schiff base Soil Spectrofluorimetric analysis

More Related Videos

A Colorimetric Method for Measuring Iron Content in Plants

A Colorimetric Method for Measuring Iron Content in Plants

Published on: September 7, 2018

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

Published on: January 31, 2022

Related Experiment Videos

Last Updated: Mar 11, 2026

Author Spotlight: Assessing the Impact of Novel Iron Chelators on Cancer Cell Metabolism

Author Spotlight: Assessing the Impact of Novel Iron Chelators on Cancer Cell Metabolism

Published on: February 23, 2024

A Colorimetric Method for Measuring Iron Content in Plants

A Colorimetric Method for Measuring Iron Content in Plants

Published on: September 7, 2018

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

Published on: January 31, 2022

Area of Science:

Analytical Chemistry
Materials Science

Background:

Iron (III) detection is vital for food safety and environmental analysis.
Existing methods for iron determination can be complex or costly.

Purpose of the Study:

To design and synthesize a chalcone-based Schiff base for iron (III) detection.
To develop a simple, cost-effective spectrofluorimetric method for iron determination in complex matrices.

Main Methods:

Synthesis of Schiff base (5) via condensation of 3-formyl-2-hydroxyquinoline and acetophenone.
Development of a spectrofluorimetric method utilizing the synthesized Schiff base.
Validation using certified reference material (CRM-SA-C Sandy Soil C) and food samples (spinach, rocket).
Sample digestion using nitric acid and hydrogen fluoride (for CRM) or nitric acid (for food) in a closed microwave system.
Measurement via modified standard addition method.

Main Results:

The Schiff base successfully detected iron (III) in partially aqueous media.
The spectrofluorimetric method demonstrated satisfactory accuracy for iron determination in spinach and rocket.
Linearity of the standard addition graph was observed up to 5.0 mg/L for iron (III).
Low detection (0.06 mg/L) and quantification (0.20 mg/L) limits were achieved.

Conclusions:

The developed Schiff base and spectrofluorimetric method are suitable for determining iron content in soil and food.
The method is simple, time-saving, cost-effective, and accurate.