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Updated: Mar 11, 2026

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
Published on: September 19, 2013
Assessing Vacuolar Escape of Listeria Monocytogenes
Juan J Quereda1,2,3, Martin Sachse4, Damien Balestrino1,2,3,5
1Institut Pasteur, Unité des Interactions Bactéries-Cellules, 25, rue du Docteur Roux, Paris, 75015, France.
Abstract:
Listeria monocytogenes is a bacterial pathogen which invades and multiplies within non-professional phagocytes. Signaling cascades involved in cellular entry have been extensively analyzed, but the events leading to vacuolar escape remain less clear. In this chapter, we detail a microscopy FRET-based assay which allows quantitatively measuring L. monocytogenes infection and escape from its internalization vacuole, as well as a correlative light/electron microscopy method to investigate the morphological features of the vacuolar compartments containing L. monocytogenes.
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