Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of the Platelet Plug01:22

Formation of the Platelet Plug

10.3K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
10.3K
Structure and Function of Platelets01:18

Structure and Function of Platelets

4.4K
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
4.4K
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

3.3K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
3.3K
Inflammation01:38

Inflammation

63.3K
Overview
63.3K
Bacterial Signaling01:30

Bacterial Signaling

42.5K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
42.5K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

17.7K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
17.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Host interactomes of <i>Streptococcus oralis</i> and <i>Streptococcus gordonii</i> exposed to saliva or serum.

Microbiology spectrum·2026
Same author

Immunomodulatory effects of eubiotic and dysbiotic multi-species biofilms on oral keratinocytes.

BMC oral health·2025
Same author

Editorial: Advancements in translational models: bridging basic infection research and clinical applications.

Frontiers in medicine·2025
Same author

The cystic fibrosis pathogen <i>Achromobacter xylosoxidans</i> inhibits biofilm formation of <i>Pseudomonas aeruginosa</i>.

Journal of medical microbiology·2025
Same author

Proteomic Profiling of Acoustically Isolated Extracellular Vesicles from Blood Plasma during Murine Bacterial Sepsis.

Journal of proteome research·2025
Same author

CpG oligonucleotides induce acute murine thrombocytopenia dependent on toll-like receptor 9 and spleen tyrosine kinase pathways.

Journal of thrombosis and haemostasis : JTH·2024

Related Experiment Video

Updated: Mar 11, 2026

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
10:10

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

Published on: October 27, 2009

18.7K

Determining Platelet Activation and Aggregation in Response to Bacteria.

Oonagh Shannon1

  • 1Division of Infection Medicine, Department of Clinical Sciences, Lund University, BMC B14, SE-221 84, Lund, Sweden. oonagh.shannon@med.lu.se.

Methods in Molecular Biology (Clifton, N.J.)
|December 4, 2016
PubMed
Summary

Pathogenic bacteria can activate human platelets, impacting infection. This study presents methods to analyze bacterial-mediated platelet activation, aggregation, and granule release for better understanding of infection pathogenesis.

Keywords:
BacteriaCoagulationFlow cytometryPlateletsStreptococci

More Related Videos

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers
05:43

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

Published on: November 8, 2024

876
Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
11:18

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Published on: April 2, 2013

16.2K

Related Experiment Videos

Last Updated: Mar 11, 2026

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
10:10

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

Published on: October 27, 2009

18.7K
An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers
05:43

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

Published on: November 8, 2024

876
Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
11:18

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Published on: April 2, 2013

16.2K

Area of Science:

  • Microbiology
  • Immunology
  • Hematology

Background:

  • Pathogenic bacteria frequently interact with human platelets.
  • The role of these bacterial-platelet interactions in infection pathogenesis and immune response remains unclear.
  • Understanding these interactions is crucial for elucidating disease mechanisms.

Purpose of the Study:

  • To develop and present methods for studying bacterial-mediated platelet responses.
  • To investigate platelet integrin activation, granule release, and aggregation induced by diverse bacterial strains.
  • To provide mechanistic insights into how bacteria influence platelet function.

Main Methods:

  • Flow cytometry was employed to assess platelet integrin activation and granule release.
  • A platelet aggregometer was utilized to measure bacterial-induced platelet aggregation.
  • A combined approach using flow cytometry and aggregometry was developed.

Main Results:

  • The described methods allow for the study of specific platelet activation pathways.
  • The combination of techniques provides a comprehensive analysis of bacterial effects on platelets.
  • These methods offer a rapid and cost-effective strategy for mechanistic investigations.

Conclusions:

  • The presented methods enable detailed characterization of platelet responses to bacterial stimuli.
  • This approach facilitates a deeper understanding of the interplay between bacteria and platelets in infection.
  • The findings contribute to clarifying the significance of bacterial-platelet interactions in pathogenesis.