Related Experiment Video

Updated: Mar 11, 2026

Laser Ablation of the Zebrafish Pronephros to Study Renal Epithelial Regeneration
07:27

Laser Ablation of the Zebrafish Pronephros to Study Renal Epithelial Regeneration

Published on: August 29, 2011

15.8K

Effects of Platelet-Rich Plasma on Kidney Regeneration in Gentamicin-Induced Nephrotoxicity

Abbas Moghadam1,2, Tahereh Talaei Khozani1, Afsaneh Mafi1,2

  • 1Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.

Journal of Korean Medical Science
|December 4, 2016
PubMed

Insights

Platelet-rich plasma (PRP) demonstrated significant kidney repair in a rat model of gentamicin-induced nephrotoxicity. PRP therapy reduced kidney fibrosis and improved tubular cell regeneration, offering a potential therapeutic strategy.

Area of Science:

  • Regenerative Medicine
  • Nephrology
  • Tissue Engineering

Background:

  • Platelet-rich plasma (PRP) contains growth factors that may promote tissue repair and reduce fibrosis.
  • Gentamicin (GM) is an antibiotic known to cause nephrotoxicity, characterized by kidney damage and fibrosis.
  • Stereological methods offer quantitative analysis of tissue structures, crucial for assessing regenerative effects.

Purpose of the Study:

  • To evaluate the therapeutic potential of PRP in ameliorating kidney damage and fibrosis induced by GM in a rat model.
  • To assess the impact of PRP on kidney regeneration using stereological techniques.
  • To investigate the effects of PRP on biochemical markers of kidney function (BUN and creatinine).

Main Methods:

  • Nephrotoxicity was induced in male rats using intraperitoneal administration of gentamicin.
  • Animals received a single intra-cortical injection of PRP (100 μL) under surgical microscopy.
  • Kidney tissues were analyzed using stereological methods (Isotropic Uniform Random sampling) and stained with Hematoxylin & Eosin and Masson's Trichrome.
  • Blood urea nitrogen (BUN) and creatinine (Cr) levels were measured before and after PRP treatment.

Main Results:

  • PRP treatment significantly increased the number of epithelial cells in convoluted tubules compared to GM-treated controls.
  • PRP administration led to a significant decrease in the volume of connective tissue within the kidney.
  • Stereological analysis showed a reduction in the volume of renal corpuscles and glomeruli following PRP therapy.
  • Biochemical markers (BUN and creatinine) showed improvement post-PRP treatment (data not detailed in abstract).

Conclusions:

  • Platelet-rich plasma exhibits beneficial effects in promoting the proliferation of renal tubular epithelial cells.
  • PRP effectively ameliorates gentamicin-induced kidney fibrosis and structural damage in a rat model.
  • These findings suggest PRP as a promising therapeutic agent for managing nephrotoxicity and promoting kidney regeneration.
Keywords:
FibrosisKidneyRegeneration

More Related Videos

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury06:25

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury

Published on: August 3, 2015

11.4K
Effects of Allogeneic Platelet-Rich Plasma PRP on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description07:51

Effects of Allogeneic Platelet-Rich Plasma PRP on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description

Published on: March 19, 2018

9.8K

Related Concept Videos

Acute Kidney Injury IV: Diagnostic Studies and Prevention01:30

Acute Kidney Injury IV: Diagnostic Studies and Prevention

Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
437
Acute Kidney Injury II: Pathophysiology01:29

Acute Kidney Injury II: Pathophysiology

Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
1.5K
Acute Kidney Injury V: Interprofessional Care01:20

Acute Kidney Injury V: Interprofessional Care

Acute Kidney Injury (AKI) requires a collaborative healthcare approach to restore renal function and prevent complications. Essential management strategies involve monitoring fluid and electrolyte balance, adjusting medications, initiating dialysis when necessary, and providing nutritional support.Fluid and Electrolyte ManagementFluid Monitoring: Regularly monitoring body weight, central venous pressure, and urine output helps detect fluid imbalances early. Patient intake and output are...
438
Acute Kidney Injury III: Clinical Manifestations01:29

Acute Kidney Injury III: Clinical Manifestations

Acute Kidney Injury (AKI) progresses through distinct clinical phases: the oliguric, diuretic, and recovery phases, each marked by unique manifestations and challenges.Oliguric Phase:The oliguric phase is the initial stage of AKI, typically lasting 10 to 14 days. This phase is marked by a significant reduction in urine output, usually less than 400 mL per day, indicating decreased kidney function. Fluid retention is a prominent feature, leading to symptoms such as edema, hypertension, and...
1.2K
Kidney Transplant II: Surgical Procedure01:26

Kidney Transplant II: Surgical Procedure

Preoperative ManagementThe primary goals of preoperative management in kidney transplantation are to optimize the patient’s metabolic state and prepare them for surgery through diet adjustments, necessary dialysis, and tailored medical treatment. This phase also involves comprehensive infection screening and patient education about the surgical procedure and postoperative care to improve outcomes and adherence.Medical ManagementA comprehensive evaluation is required for both the living...
628