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Updated: Mar 10, 2026
14:18
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
Published on: February 13, 2012
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Store operated calcium entry: From concept to structural mechanisms
Peter B Stathopulos1, Mitsuhiko Ikura2
1Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada.
Cell Calcium
|December 5, 2016
Summary
Store-operated calcium entry (SOCE) is crucial for cell signaling. Recent structural biology research illuminates the molecular mechanisms regulating this vital calcium influx process, building on foundational models.
Area of Science:
- Cell Biology
- Biochemistry
- Structural Biology
Background:
- The 1986 model by J.W. Putney proposed that endoplasmic reticulum calcium depletion activates plasma membrane calcium channels.
- This process, known as store-operated calcium entry (SOCE), is essential for numerous cellular signaling pathways.
- Dysregulation of SOCE is implicated in various diseases.
Purpose of the Study:
- To review known structures related to SOCE regulation.
- To highlight the atomic mechanisms governing SOCE.
- To discuss future research directions in the field.
Main Methods:
- This review synthesizes findings from structural biology research.
- It focuses on known protein structures involved in SOCE.
- The review discusses mechanisms derived from these structures.
Main Results:
- Key biomolecules regulating SOCE are now identified.
- Structural insights are beginning to reveal the atomic basis of SOCE regulation.
- Understanding these structures is critical for elucidating SOCE function.
Conclusions:
- Structural biology has significantly advanced the understanding of SOCE mechanisms.
- Future research will likely focus on refining these structural models and their functional implications.
- Putney's conceptualization remains a cornerstone for current SOCE research.
Keywords:
Crystal structureNMR StructureOrai1StoichiometryStore operated calcium entryStromal interaction moleculesMore Related Videos
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