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Related Experiment Video

Updated: Mar 10, 2026

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
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Intra-laboratory validated human cell-based in vitro vasculogenesis/angiogenesis test with serum-free medium.

T Toimela1, O Huttala1, E Sabell1

  • 1FICAM, University of Tampere, Finland.

Reproductive Toxicology (Elmsford, N.Y.)
|December 5, 2016
PubMed
Summary

A new human cell-based test accurately identifies chemicals that may inhibit blood vessel formation (vasculogenesis/angiogenesis), aiding in the prediction of developmental toxicity.

Keywords:
AngiogenesisChemical testingHuman adipose stromal cellsHuman umbilical cord vein endothelial cellsIn vitroTeratogensVasculogenesis

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Area of Science:

  • Biomedical science
  • Toxicology
  • Developmental biology

Background:

  • Vasculogenesis and angiogenesis are critical for new blood vessel formation.
  • Understanding these processes is vital for assessing chemical safety.
  • Existing methods for evaluating developmental toxicity can be limited.

Purpose of the Study:

  • To validate a novel serum-free human cell-based assay for assessing vasculogenesis and angiogenesis.
  • To evaluate the test's reproducibility, repeatability, and predictive value for human developmental toxicity.
  • To determine if inhibition of vasculogenesis is a key mechanism in teratogenesis.

Main Methods:

  • Utilized human adipose stromal cells and umbilical cord vein endothelial cells in a serum-free assay.
  • Validated the test in a Good Laboratory Practice (GLP) laboratory following OECD guidelines.
  • Assessed tubule formation via immunostaining and performed cytotoxicity evaluations.
  • Tested the assay's performance using 26 known teratogens and non-teratogens.

Main Results:

  • The assay demonstrated reproducibility and repeatability.
  • The test achieved a positive predictive value of 71.4% and a negative predictive value of 50.0% for teratogenicity.
  • Results indicate that inhibition of vasculogenesis is a significant factor in chemical-induced developmental harm.

Conclusions:

  • The developed human cell-based assay shows significant potential for screening chemicals.
  • This test can aid in prioritizing substances for further developmental toxicity testing.
  • The assay may serve as a valuable component in regulatory testing batteries for predicting developmental hazards.