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Modification of orthogonal tRNAs: unexpected consequences for sense codon reassignment

Wil Biddle1, Margaret A Schmitt2, John D Fisk3,2,4

  • 1Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA.

Nucleic Acids Research
|December 5, 2016
PubMed
Summary

Genetic code engineering can incorporate non-canonical amino acids. We found a tRNA modification that unexpectedly altered codon recognition, impacting protein synthesis and offering new strategies for genetic code expansion.

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