Related Experiment Video
Updated: Mar 10, 2026
An In Ovo Model for Testing Insulin-mimetic Compounds
Published on: April 23, 2018
Integrating insulin into single-step culture medium regulates human embryo development in vitro
Mohamed Fawzy1, Mohamed Sabry2, Mohamed Nour2
1IbnSina IVF Center, IbnSina Hospital, Sohag, Egypt.
Objective:
To evaluate the effect of supplementing single-step embryo culture medium with insulin on human embryo development.
Design:
Comparative study.
Setting:
Two private centers.
Patient(S):
The study involved a sibling oocyte split of 5,142 retrieved oocytes from 360 patients.
Intervention(S):
Sibling oocytes split after intracytoplasmic sperm injection for culture from day 0 through day 5 or 6 in insulin-supplemented or control medium. Women were split to receive their embryos from insulin-supplemented or control medium.
Main Outcome Measure(S):
Clinical pregnancy rate.
Result(S):
There were significantly higher rates of clinical, ongoing, and twin pregnancies in the insulin-supplemented arm than in the control arm. On day 3, embryo quality and compaction were higher in insulin-supplemented medium. On day 5, insulin supplementation showed higher rates of blastocyst formation, quality, and cryopreservation.
Conclusion(S):
Insulin supplementation of single-step embryo culture medium from day 0 through day 5 or 6 improved clinical pregnancy rate and human embryo development. However, these findings need further confirmation through a multicenter randomized controlled trial that may include other patient populations and different culture media.
More Related Videos
05:13Author Spotlight: Advancing Therapeutic Strategies for Improving Pregnancy Rates by Analyzing Embryo-Endometrium Interactions
Published on: June 21, 2024
08:29Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach
Published on: March 27, 2012
