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Translational Regulation01:29

Translational Regulation

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Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
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Equipments Used to Measure Body Temperature01:13

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Body temperature can be assessed using various devices and measured in Celsius or Fahrenheit.
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
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Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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Thermosensation01:43

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Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
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Temperature Measurement Sites01:14

Temperature Measurement Sites

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A thermometer measures body temperature. The common sites for measuring body temperature are the oral cavity, axillary region, temporal artery, and skin surface, such as the forehead, abdomen, and axilla. True core body temperature is assessed in the rectum, tympanic membrane, pulmonary artery, esophagus, and urinary bladder.
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Assessing Body Temperature - Tympanic membrane01:14

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Assessing tympanic membrane temperature involves using a tympanic membrane thermometer (TMT). Here is a step-by-step guide:
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Understanding thermal sensitivity at the molecular level and developing temperature-based systems using RNA

Fatih Gul1, Ibrahim Y Orhan, Furkan S Ceylan

  • 1. fatihgtlos@gmail.com.

Clinical and Investigative Medicine. Medecine Clinique Et Experimentale
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Summary

This study investigates RNA Thermometers for temperature-dependent gene regulation. A novel automated system using enzymatic reactions was proposed for precise heat stabilization in biological systems.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Systems Biology

Background:

  • Temperature sensitivity is a fundamental biological property observed across all multicellular organisms and primitive life forms.
  • Temperature regulation impacts biological systems at multiple levels: multicellular, cellular, and molecular.
  • RNA Thermometers, which are temperature-sensitive RNA sequences, are known to regulate heat-shock genes and temperature-dependent processes.

Purpose of the Study:

  • To characterize the functional changes of RNA Thermometers at different temperatures.
  • To propose an automated temperature-responsive system based on transcriptional thermoregulation.
  • To stimulate inverse endothermic and exothermic enzymatic reactions for effective heat stabilization.

Main Methods:

  • Designated the endothermic reaction as urea breakdown (urease function) and the exothermic reaction as hydrogen peroxide breakdown (catalase function).
  • Developed a system based on temperature-dependent translation of urease and inhibition of catalase.
  • Incorporated a lac-regulated 2-way system to enable RNA Thermometer function at higher temperatures.

Main Results:

  • The proposed system demonstrates temperature-responsive control through differential enzyme activity.
  • The integration of a lac-regulated system allows for transcriptional control at elevated temperatures.
  • The system offers a synthetic approach to thermoregulation, potentially improving upon current methods.

Conclusions:

  • RNA Thermometers can be leveraged for sophisticated temperature-based gene regulation.
  • The proposed automated system offers a novel synthetic solution for thermoregulation.
  • This system has the potential to be optimized and applied in scenarios where current thermoregulatory systems are insufficient.