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Lytic Activity of Various Phage Cocktails on Multidrug-Resistant Bacteria

I Ozkan1, E Akturk, N Yeshenkulov

  • 1. ismail.ozkan@sifa.edu.tr.

Clinical and Investigative Medicine. Medecine Clinique Et Experimentale
|December 6, 2016
PubMed
Abstract

Insights

Bacteriophage cocktails, including Pyo, Intesti, and Fersisi, show high effectiveness against multidrug-resistant Staphylococcus aureus and Pseudomonas aeruginosa clinical strains. These cocktails offer a promising therapeutic approach for infections caused by these challenging bacteria.

Area of Science:

  • Microbiology
  • Virology
  • Infectious Diseases

Background:

  • Bacteriophages are viruses that infect bacteria and are explored as alternatives to antibiotics.
  • Multi-drug resistant (MDR) bacterial infections pose a significant global health threat.
  • Phage therapy offers a potential solution for combating antibiotic-resistant pathogens.

Purpose of the Study:

  • To evaluate the lytic activity of Pyo, Intesti, and Fersisi bacteriophage cocktails.
  • To assess the efficacy of these cocktails against clinical isolates of P. aeruginosa and S. aureus.
  • To determine the potential of phage cocktails as a treatment for MDR bacterial infections.

Main Methods:

  • Ten clinical isolates of S. aureus and P. aeruginosa from Turkey were used.
  • Bacterial identification and antibiotic susceptibility were determined using the Vitec 2 system.
  • Species-specific PCR confirmed isolate identities, and lytic activity was assessed via spot tests and plaque assays.

Main Results:

  • The Pyo phage cocktail demonstrated susceptibility in eight MDR S. aureus isolates and nine antibiotic-resistant P. aeruginosa isolates.
  • Only two S. aureus and one P. aeruginosa isolate showed resistance to the Pyo cocktail.
  • The Pyo, Intesti, and Fersisi cocktails proved highly effective against Turkish clinical strains of MDR P. aeruginosa and S. aureus.

Conclusions:

  • Bacteriophage cocktails (Pyo, Intesti, Fersisi) are effective against MDR P. aeruginosa and S. aureus.
  • These phage cocktails represent a viable therapeutic option for infections caused by these bacteria.
  • Further research into phage therapy is warranted for treating multidrug-resistant bacterial infections.

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