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Updated: Mar 10, 2026

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
Published on: April 22, 2022
The 5-chlorouracil:7-deazaadenine base pair as an alternative to the dT:dA base pair
E Eremeeva1, M Abramov1, P Marlière2
1KU Leuven, Rega Institute for Medical Research, Medicinal Chemistry, Minderbroedersstraat 10, BE-3000 Leuven, Belgium. piet.herdewijn@kuleuven.be.
Abstract:
5-Chloro-2'-deoxyuridine as a possible component of a chemically modified genome has been discussed in terms of its influence on duplex stability and DNA polymerase incorporation properties. The search for its counterpart among different deoxyadenosine analogs (7-deaza-, 8-aza- and 8-aza-7-deaza-2'-deoxyadenosines) showed that the stable duplex formation as well as the synthesis of long constructs, more than 2 kb, were successful with the 5-chloro-2'-deoxyuridine and 7-deaza-2'-deoxyadenosine combination and with Taq DNA polymerase.
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