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Novel rat tail discitis model using bioluminescent Staphylococcus aureus.

Phillip A Bostian1, Jonathan M Karnes1, Shari Cui1

  • 1Department of Orthopaedics, West Virginia University, P.O. Box 9196, Morgantown, West Virginia 26506-9196.

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Summary

This study developed a rat model for spondylodiscitis using bioluminescent Staphylococcus aureus. The model allows real-time infection monitoring, potentially reducing animal use in spinal infection research.

Keywords:
bioluminescencediscitisinfectionintervertebral discspine

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Area of Science:

  • Orthopaedic Research
  • Infectious Disease Modeling
  • Biotechnology

Background:

  • Spondylodiscitis management presents significant clinical challenges.
  • Effective medical and surgical treatments are crucial.
  • A reliable animal model is needed for research.

Purpose of the Study:

  • To establish a novel rat model for spondylodiscitis.
  • Utilize bioluminescent Staphylococcus aureus (S. aureus) for in vivo infection surveillance.
  • Enable real-time quantification of infection intensity.

Main Methods:

  • Injected varying concentrations of bioluminescent S. aureus (XEN36) into rat tail intervertebral discs.
  • Used saline as a control.
  • Monitored bioluminescence for 6 weeks, alongside radiographic and histologic analyses.

Main Results:

  • Bioluminescence peaked at day 3 and normalized within 21 days.
  • Highest intensity observed with the 10^6 CFU/0.1ml S. aureus concentration.
  • Radiography and histology confirmed disc space loss, vertebral endplate destruction, and osseous bridging.

Conclusions:

  • Injection of bioluminescent S. aureus in rats is a viable model for spondylodiscitis.
  • This model allows in vivo quantification of infection.
  • The model may reduce the number of animals needed for intervertebral disc infection studies.