Carbon dioxide insufflation deflects airborne particles from an open surgical wound model

Surgical site infections remain a significant burden on healthcare systems and may benefit from new countermeasures.

To assess the merits of open surgical wound CO₂ insufflation via a gas diffuser to reduce airborne contamination, and to determine the distribution of CO₂ in and over a wound.

An experimental approach with engineers and clinical researchers was employed to measure the gas flow pattern and motion of airborne particles in a model of an open surgical wound in a simulated theatre setting. Laser-illuminated flow visualizations were performed and the degree of protection was quantified by collecting and characterizing particles deposited in and outside the wound cavity.

The average number of particles entering the wound with a diameter of <5μm was reduced 1000-fold with 10L/min CO₂ insufflation. Larger and heavier particles had a greater penetration potential and were reduced by a factor of 20. The degree of protection was found to be unaffected by exaggerated movements of hands in and out of the wound cavity. The steady-state CO₂ concentration within the majority of the wound cavity was >95% and diminished rapidly above the wound to an atmospheric level (∼0%) at a height of 25mm.

Airborne particles were deflected from entering the wound by the CO₂ in the cavity akin to a protective barrier. Insufflation of CO₂ may be an effective means of reducing intraoperative infection rates in open surgeries.