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Related Experiment Videos

The spatial and temporal expression pattern of sevenless is exclusively controlled by gene-internal elements.

K Basler1, P Siegrist, E Hafen

  • 1Zoologisches Institut, Universität Zürich, Switzerland.

The EMBO Journal
|August 1, 1989
PubMed
Summary

Regulatory elements controlling the sevenless gene are located 3' to its transcription start site. These internal sequences drive the gene's expression pattern in the developing Drosophila eye.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • The sevenless gene is crucial for specifying a specific photoreceptor cell type during Drosophila eye development.
  • Sevenless RNA and protein exhibit transient accumulation in a subset of cells within the developing eye imaginal disc.

Purpose of the Study:

  • To identify the cis-acting regulatory regions responsible for the intricate sevenless expression pattern.
  • To elucidate the role of upstream and downstream sequences in sevenless gene regulation.

Main Methods:

  • Utilized P-element transformation with various minigenes and marker gene constructs.
  • Analyzed the function of gene-internal sequences on heterologous promoters and as reporter gene elements.

Main Results:

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  • The upstream region of the sevenless gene lacks detectable regulatory elements.
  • Sequences located 3' to the transcription start site are sufficient for directing the sevenless expression pattern.
  • These internal regulatory sequences function independently of orientation and position relative to a reporter gene.

Conclusions:

  • Gene regulatory elements controlling sevenless expression are located within the gene body, specifically downstream of the transcription start site.
  • These findings provide insights into the complex regulation of cell fate determination during eye development.