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Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
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Digital PCR: A brief history.

Alexander A Morley1

  • 1Department of Haematology and Genetic Pathology, Flinders University and Medical Centre, Bedford Park, SA 5042, Australia.

Biomolecular Detection and Quantification
|December 7, 2016
PubMed
Summary
This summary is machine-generated.

Digital PCR, a method for quantifying targets, has evolved significantly since its inception. Recent advancements in instrumentation and chemistry have revitalized digital PCR, making it more accessible and practical for researchers.

Keywords:
Digital PCRLimiting dilutionPCR

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genomics

Background:

  • Digital PCR (dPCR) was first described as "limiting dilution PCR" in 1990-1991 and later as "digital PCR" in 1999.
  • Its initial adoption was limited by the emergence of real-time PCR in 1996.
  • dPCR has experienced a resurgence due to recent technological innovations.

Approach:

  • The technique involves partitioning a sample into numerous small reaction volumes.
  • Each partition is analyzed for the presence or absence of the target DNA.
  • Quantification is achieved by counting the positive partitions, enabling absolute quantification.

Key Points:

  • Digital PCR offers high precision and sensitivity for nucleic acid quantification.
  • Recent developments have simplified dPCR workflows and improved its practicality.
  • This technique is valuable for applications such as rare mutation detection and copy number variation analysis.

Conclusions:

  • Digital PCR has undergone significant development and refinement.
  • Modern instrumentation and chemistry have overcome previous limitations, enhancing its usability.
  • The renewed accessibility of digital PCR positions it as a powerful tool in molecular diagnostics and research.