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Related Experiment Videos

Elevated temperature regulates tumor necrosis factor-mediated immune killing.

S H Gromkowski1, J Yagi, C A Janeway

  • 1Section of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510.

European Journal of Immunology
|September 1, 1989
PubMed
Summary

Elevated temperatures, like fever, temporarily reduce the immune system's ability to kill cells via tumor necrosis factor (TNF). Heat shock also prevents inflammatory T cells from secreting TNF or killing target cells.

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Tumor necrosis factor (TNF) is a key mediator in inflammation and can induce cell death.
  • The relationship between heat shock and TNF-mediated cytotoxicity is not fully understood.
  • Understanding how temperature affects inflammatory processes is crucial for immune response research.

Purpose of the Study:

  • To investigate the effect of elevated temperature on TNF-mediated cell lysis.
  • To examine the impact of heat shock on the sensitivity of tumor cells to TNF.
  • To determine how heat affects the function of inflammatory T lymphocytes.

Main Methods:

  • In vitro study using WEHI-164 fibrosarcoma cells and inflammatory T lymphocytes.
  • Exposure of cells to elevated temperatures (heat shock).

Related Experiment Videos

  • Assessment of cell sensitivity to recombinant TNF and T cell-mediated lysis.
  • Measurement of TNF secretion by T lymphocytes.

    • Main Results:

    • Heat shock transiently decreased WEHI-164 cell sensitivity to TNF-mediated lysis.
    • Heat shock also reduced sensitivity to lysis mediated by TNF-secreting T cells.
    • Elevated temperatures transiently abolished the lytic potential of inflammatory T lymphocytes.
    • Heat shock temporarily inhibited TNF secretion by effector T lymphocytes.

    Conclusions:

    • Elevated temperatures can modulate inflammatory responses by affecting TNF signaling.
    • Heat shock protects target cells from TNF-induced death by inducing protective proteins.
    • Heat shock can arrest TNF secretion by effector T lymphocytes, thereby controlling cytolytic processes.
    • Temperature plays a regulatory role in inflammation and immune cell function.