Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 10, 2026

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix
10:48

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix

Published on: November 13, 2023

1.7K

ClC-3 Promotes Osteogenic Differentiation in MC3T3-E1 Cell After Dynamic Compression.

Dawei Wang1, Hao Wang1, Feng Gao2

  • 1Department of Stomatology, Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei, China.

Journal of Cellular Biochemistry
|December 7, 2016
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Can increased cognitive load help people with subthreshold depression to forget negative information?

Journal of affective disorders·2021
Same author

Vacuolin-1 inhibits endosomal trafficking and metastasis via CapZβ.

Oncogene·2021
Same author

CTPA pulmonary artery distensibility in assessment of severity of acute pulmonary embolism and right ventricular function.

Medicine·2021
Same author

The complete chloroplast genome of <i>Castanopsis mekongensis</i> A. Camus (Fagaceae).

Mitochondrial DNA. Part B, Resources·2021
Same author

3D images of the silicone implants in capsular contracture after breast augmentation using magnetic resonance imaging with SPACE sequence.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2021
Same author

High-efficient liquid exfoliation of 2D metal-organic framework using deep-eutectic solvents.

Ultrasonics sonochemistry·2021

Dynamic compression upregulates ClC-3 chloride channels, promoting osteogenic differentiation in MC3T3-E1 cells. Inhibiting ClC-3 reduces key osteogenic marker expression, suggesting ClC-3

Area of Science:

  • Biochemistry and Molecular Biology
  • Cell Biology
  • Biomaterials Science

Background:

  • ClC-3 chloride channels are implicated in osteogenesis and osteoblast differentiation.
  • Static compression upregulates ClC-3, but its role under dynamic compression is unknown.

Purpose of the Study:

  • To investigate the role of ClC-3 in osteogenic differentiation of MC3T3-E1 cells under dynamic compression.
  • To examine the expression of osteogenic markers and proliferation genes following dynamic compression.

Main Methods:

  • MC3T3-E1 cells were subjected to dynamic compression in a biopress system.
  • Gene expression of ClC-3, Runx2, BMP-2, OPN, Ki67, and PCNA was analyzed.
  • Transmission electron microscopy was used to observe cellular ultrastructure.
Keywords:
ClC-3 CHLORIDE CHANNElDYNAMIC COMPRESSIONOSTEOGENIC DIFFERENTIATIONTHREE-DIMENSIONAL-OSTEOBLASTS MEMBRANE

More Related Videos

A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling
08:28

A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling

Published on: May 21, 2020

7.6K
A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression
07:23

A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression

Published on: September 13, 2019

7.2K

Related Experiment Videos

Last Updated: Mar 10, 2026

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix
10:48

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix

Published on: November 13, 2023

1.7K
A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling
08:28

A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling

Published on: May 21, 2020

7.6K
A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression
07:23

A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression

Published on: September 13, 2019

7.2K
  • Chlorotoxin, a ClC-3 inhibitor, was used to assess its impact on gene expression.

    • Main Results:

    • Dynamic compression significantly upregulated ClC-3 expression in MC3T3-E1 cells.
    • Osteogenic markers Runx2, BMP-2, and OPN were upregulated by dynamic compression.
    • Cell proliferation markers Ki67 and PCNA showed no significant change after 8 hours of compression.
    • Chlorotoxin treatment reduced Runx2, BMP-2, and OPN expression without affecting ClC-3 levels.

    Conclusions:

    • ClC-3 chloride channels play a crucial role in promoting osteogenic differentiation in MC3T3-E1 cells under dynamic compression.
    • Dynamic compression influences cellular morphology and promotes osteogenesis via ClC-3.
    • Targeting ClC-3 may offer a therapeutic strategy for bone regeneration.