Structural analysis of lens epithelial explants induced to differentiate into fibres by fibroblast growth factor...

Recently we identified fibroblast growth factor (FGF), which is present in significant amounts in neural retinas, as a potent inducer of lens fibre differentiation in our epithelial explant cultures. Fibre differentiation was assessed by synthesis of fibre specific, proteins, beta- and gamma-crystallins, and by cell elongation. However, to establish whether FGF induced the dramatic structural changes characteristic of fibre differentiation we carried out an ultrastructural analysis. In this study epithelial explants exposed to either the acidic or basic form of FGF were shown to undergo the structural changes characteristic of fibre differentiation in the intact lens. These include: (i) cell elongation, (ii) a reduction in cytoplasmic organelles, (iii) the formation of specialized cell-cell junctions, including finger-like processes and fingerprints, ball and socket junctions, tongue-like flaps and imprints, and gap junctions, and (iv) nuclear pyknosis. This shows that FGF faithfully reproduces structural events associated with fibre differentiation as well as the molecular events reported previously, thus providing further evidence that FGF in the eye is important for the control of normal lens fibre differentiation.