Related Experiment Video
Updated: Mar 10, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
The intricate dance of post-translational modifications in the rhythm of life
Arisa Hirano1, Ying-Hui Fu1, Louis J Ptáček1
1Kavli Institute for Fundamental Neuroscience; Weill Institute of Neurosciences; Howard Hughes Medical Institute; and Department of Neurology, University of California, San Francisco, San Francisco, California USA.
Abstract:
Endogenous biological rhythms with approximately 24-h periodicity are generated by the circadian clock, in which clock genes coordinate with one another and form a transcriptional-translational negative feedback loop. The precision of the circadian clock is further regulated by multiple post-translational modifications (PTMs), including phosphorylation, ubiquitination, acetylation and SUMOylation. Here, we review current understanding of the regulatory mechanisms of the core clock proteins by PTMs in the mammalian circadian clock.
More Related Videos
08:12Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
Published on: January 8, 2018
09:29Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
Related Concept Videos
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein....
Covalently Linked Protein Regulators
Regulation of Expression at Multiple Steps
Bacterial Protein Maturation
Protein Modifications in the RER
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...