Related Experiment Video
Updated: Mar 10, 2026
A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms
Published on: September 16, 2022
Plant extracts: initial screening, identification of bioactive compounds and effect against Candida albicans biofilms
Fernanda Lourenção Brighenti1, Marcos José Salvador2, Aline Vidal Lacerda Gontijo2
1Faculty of Dentistry, Univ. Estadual Paulista/UNESP, Araraquara, R. Humaitá, 1680, Araraquara, SP 14801-903, Brazil.
Aim:
This study screened plants from Brazilian Pantanal for Candida albicans antibiofilm activity.
Material & Methods:
Sixty extracts were obtained from ten plants using different extraction methods. Antifungal activity was assessed. Effects on biofilm inhibition and disruption and cytotoxicity were also evaluated. The most active extract was chemically characterized.
Results:
Buchenavia tomentosa ethanolic extract showed noticeable antifungal activity and was selected for biofilm experiments. Subinhibitory concentration of extract inhibited fungal adhesion. Maximum killing reached 90% of C. albicans cells in suspension and 65% of cells in biofilms. The active extract was noncytotoxic. Chemical characterization showed the presence of phenols. Ellagic and gallic acids showed activity on C. albicans.
Conclusion:
B. tomentosa extract and its isolated compound, ellagic acid, presented antibiofilm activity and low toxicity.
