Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

10.2K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.2K
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

10.1K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
10.1K
Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

9.3K
Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
9.3K
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

7.8K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
7.8K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.9K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.9K
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

88.0K
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
88.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial Expression of Concern: DOCK8 functions as an adaptor that links TLR-MyD88 signaling to B cell activation.

Nature immunology·2026
Same author

Long-term outcome in Wiskott-Aldrich syndrome and X-linked thrombocytopenia patients: an observational -prospective multi-center study of the Italian Primary Immune Deficiency Network (IPINET).

EClinicalMedicine·2025
Same author

A precision medicine approach to primary immunodeficiency disease: Ataluren strikes nonsense mutations once again.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

Correction: The intestinal microbiome and metabolome discern disease severity in cytotoxic T-lymphocyte-associated protein 4 deficiency.

Microbiome·2025
Same author

Uncovering Risk Factors of Premature Mortality in Common Variable Immunodeficiency.

The journal of allergy and clinical immunology. In practice·2025
Same author

The intestinal microbiome and metabolome discern disease severity in cytotoxic T-lymphocyte-associated protein 4 deficiency.

Microbiome·2025

Related Experiment Video

Updated: Mar 10, 2026

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
10:36

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

Published on: January 9, 2018

7.6K

NFKB1 regulates human NK cell maturation and effector functions.

Vassilios Lougaris1, Ornella Patrizi2, Manuela Baronio1

  • 1Pediatrics Clinic and Institute for Molecular Medicine A. Nocivelli, Department of Clinical and Experimental Sciences, University of Brescia, Spedali Civili di Brescia, Italy.

Clinical Immunology (Orlando, Fla.)
|December 8, 2016
PubMed
Summary

Mutations in NFKB1 impair natural killer (NK) cell maturation and function. These findings reveal a novel role for NFKB1 in human NK cell biology and immune responses.

Keywords:
CytotoxicityInterferon-γNFKB1Natural killer cells

More Related Videos

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
06:55

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

Published on: February 22, 2020

19.6K
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

9.6K

Related Experiment Videos

Last Updated: Mar 10, 2026

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
10:36

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

Published on: January 9, 2018

7.6K
Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
06:55

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

Published on: February 22, 2020

19.6K
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

9.6K

Area of Science:

  • Immunology
  • Cell Biology
  • Genetics

Background:

  • NFKB1 is a component of the canonical NF-κB pathway.
  • Mutations in NFKB2 have been linked to impaired NK cell activity.
  • The role of NFKB1 mutations in human NK cells is not well understood.

Purpose of the Study:

  • To investigate the role of monoallelic NFKB1 mutations in human NK cell maturation and function.

Main Methods:

  • Evaluation of NK cell maturation and function in patients with NFKB1 mutations.
  • In vitro stimulation of NK cells and assessment of cytotoxicity, IFN-γ production, NKp44 expression, and proliferation.

Main Results:

  • NFKB1-mutated NK cells exhibit impaired maturation.
  • Defective cytotoxicity and reduced IFN-γ production were observed in NFKB1-mutated NK cells.
  • IL-2 activated NFKB1-mutated NK cells showed reduced NKp44 upregulation and proliferation.

Conclusions:

  • NFKB1 plays a critical role in human NK cell maturation and effector functions.
  • NFKB1 mutations impact multiple aspects of NK cell biology.
  • These findings highlight NFKB1 as a potential target for immune-related disorders.