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Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3
M Kriechbaum1, H J Heilmann, F J Wientjes
1Institut für Biochemie, Technische Hochschule Darmstadt, FRG.
Abstract:
A cDNA library from Aspergillus niger strain NRRL-3 enriched in sequences glucose oxidase was constructed. An 800 bp cDNA clone isolated from this library was used to screen 12,000 recombinant phages from an EMBL3 genomic library. A 15 kbp DNA segment isolated from this library contained the 1815 bp structural gene for glucose oxidase as well as a short 5'- and a longer 3'-noncoding region. The deduced protein sequence was verified by partial peptide sequencing.
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