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The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
Published on: April 10, 2015
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Correlation Between Molecular Modelling and Spectroscopic Techniques in Investigation With DNA Binding Interaction of
B Thulasiram1, C Shobha Devi2, Yata Praveen Kumar3
1Inorganic & Physical Chemistry Division, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad, 500007, Telangana State, India.
Journal of Fluorescence
|December 8, 2016
Summary
Ruthenium(II) polypyridyl complexes bind to DNA via intercalation, forming bonds with nucleotide base pairs. Molecular modeling confirmed experimental findings on these DNA-binding interactions.
Area of Science:
- Coordination Chemistry
- Bioinorganic Chemistry
- Molecular Interactions
Background:
- Ruthenium(II) polypyridyl complexes are investigated for their potential interactions with DNA.
- Understanding these interactions is crucial for developing novel therapeutic or diagnostic agents.
Purpose of the Study:
- To investigate the DNA binding modes of various ruthenium(II) polypyridyl complexes.
- To elucidate the binding interactions using spectroscopic and computational methods.
Main Methods:
- Electronic absorption titration and emission spectroscopy were employed to study DNA binding.
- Molecular modeling and docking studies were performed to visualize and analyze the binding interactions.
Main Results:
- Ruthenium(II) complexes exhibited strong binding affinities to DNA, with binding constants in the range of 10^4 to 10^5.
- The complexes predominantly bind to DNA through intercalation, facilitated by their planar ligands.
- Ancillary ligands (phen, dmb, bpy) were found to interact with the DNA phosphate backbone.
Conclusions:
- The study successfully characterized the DNA binding behavior of ruthenium(II) polypyridyl complexes.
- Experimental and computational results showed good agreement, validating the proposed binding modes.
- Molecular modeling provides valuable insights into the nature and specificity of ruthenium(II) complex-DNA interactions.
