Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

17.5K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
17.5K
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase01:11

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

60
Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
60
Pharmacogenomics: Identification of New Drug Targets01:29

Pharmacogenomics: Identification of New Drug Targets

57
Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...
57
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

820
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
820

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct volatile profiles of Metschnikowia pulcherrima L672 and Hanseniaspora uvarum L793 determine their antagonistic efficacy against Aspergillus flavus in a dried fig agar model.

International journal of food microbiology·2026
Same author

Microbial Detoxification of Ochratoxin A in Food.

Foods (Basel, Switzerland)·2026
Same author

Preharvest application of antagonistic yeasts for the control of fungal pathogens in organic peach and plum orchards.

Journal of the science of food and agriculture·2026
Same author

Effects of Preharvest Application of Oxalic Acid, γ-Aminobutyric Acid, and Melatonin on the Microbiological and Physicochemical Quality of Dried Figs at Commercial Harvest and During Storage.

Toxins·2026
Same author

Optimization of Bioactive Lipid Synthesis by Enzymatic Acidolysis Using EPA + DHA Concentrate from Rainbow Trout and Tocopherols from Maqui Seed Oil.

Foods (Basel, Switzerland)·2026
Same author

Lactic Acid Bacteria Isolated from Traditional Dry-Cured Fermented Foods with Probiotic Effect: Selection, Mechanisms of Action and Applications.

Foods (Basel, Switzerland)·2025

Related Experiment Video

Updated: Mar 10, 2026

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
09:21

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

11.3K

Targeting Other Mycotoxin Biosynthetic Genes.

María J Andrade1, Mar Rodríguez2, Juan J Córdoba2

  • 1Faculty of Veterinary Science, Food Hygiene and Safety, Meat and Meat Products Research Institute, University of Extremadura, Avda. delas Ciencias s/n, 10003, Cáceres, Spain. mjandrad@unex.es.

Methods in Molecular Biology (Clifton, N.J.)
|December 8, 2016
PubMed
Summary

Real-time PCR (qPCR) offers sensitive detection of food-contaminating toxigenic molds. Protocols are detailed for quantifying molds producing zearalenone, sterigmatocystin, cyclopiazonic acid, and patulin in foods.

Keywords:
Biosynthesis pathway genesCyclopiazonic acidMoldsPatulinSterigmatocystinZearalenoneqPCR

More Related Videos

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
09:44

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

Published on: December 21, 2015

21.6K
Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
09:52

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Published on: July 12, 2013

17.7K

Related Experiment Videos

Last Updated: Mar 10, 2026

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
09:21

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

11.3K
RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
09:44

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

Published on: December 21, 2015

21.6K
Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
09:52

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Published on: July 12, 2013

17.7K

Area of Science:

  • Food microbiology
  • Molecular biology
  • Mycotoxicology

Background:

  • Toxigenic molds contaminate food commodities, posing health risks.
  • Accurate quantification of these molds is crucial for food safety.
  • Existing detection methods may lack sensitivity or speed.

Purpose of the Study:

  • To describe reliable quantitative real-time PCR (qPCR) protocols for detecting and quantifying specific toxigenic molds.
  • To provide methods applicable to both pure mold cultures and contaminated food samples.
  • To detail protocols for molds producing key mycotoxins: zearalenone (ZEA), sterigmatocystin (ST), cyclopiazonic acid (CPA), and patulin (PAT).

Main Methods:

  • Development and description of qPCR assays targeting specific mycotoxin biosynthesis genes.
  • Utilization of SYBR Green and TaqMan methodologies for quantification.
  • Inclusion of a competitive internal amplification control for CPA-producing molds.
  • A standardized DNA extraction protocol for mold cultures and food matrices.

Main Results:

  • Established qPCR protocols for sensitive and rapid detection and quantification of ZEA-, ST-, CPA-, and PAT-producing molds.
  • Demonstrated suitability of the methods for both pure cultures and complex food matrices.
  • Validated specific gene targets (PKS genes for ZEA, fluG for ST, idh for PAT) for qPCR quantification.
  • Provided a robust DNA extraction method critical for accurate qPCR results.

Conclusions:

  • qPCR is an effective tool for sensitive and rapid detection and quantification of toxigenic molds in food.
  • The described protocols offer reliable methods for monitoring mycotoxin-producing fungi.
  • Standardized DNA extraction is essential for successful qPCR-based mold quantification in food safety applications.