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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Updated: Mar 10, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

Proteomic Analysis of Dental Tissue Microsamples.

Jonathan E Mangum¹, Jew C Kon^1,2, Michael J Hubbard^3,4

¹Department of Pharmacology and Therapeutics, University of Melbourne, Melbourne, VIC, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|December 8, 2016

Summary

This study presents refined gel-based proteomic methods for analyzing proteins in developing rat and mouse teeth. These techniques are crucial for understanding dental enamel development and can be applied to scarce samples, including human enamel defects.

Keywords:

Ameloblast Dental development Enamel defects Functional proteomics Gel electrophoresis Microsample proteomics Rat and mouse models Sample preparation

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Area of Science:

Biomedical research
Dental science
Proteomics

Background:

Understanding dental enamel development is vital for both dentistry and broader biomedical applications.
Rat and mouse models are valuable for studying enamel development but present challenges for proteomic analysis due to their small size.

Purpose of the Study:

To develop and describe gel-based proteomic methods for analyzing proteins in developing rat and mouse teeth.
To enable parallel quantification, identification, and functional characterization of tooth proteins from scarce samples.

Main Methods:

Gel-based proteomic techniques were refined for small-scale sample analysis.
Methods allow for parallel quantification, identification, and functional characterization of proteins.

Main Results:

Successfully applied refined proteomic methods to analyze proteins in developing rat and mouse teeth.
Demonstrated the utility of these methods for scarce biological samples.

Conclusions:

The described gel-based proteomic methods enhance the study of dental enamel development in rodent models.
These refined techniques are applicable to challenging samples, such as human enamel defects, advancing biomedical and dental research.