Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

21.7K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
21.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Research on the Rapid Detection of Pesticide Residues in Vegetables Based on Magnetic Bead Purification.

Journal of agricultural and food chemistry·2026
Same author

Non-Targeted Screening and Quantitative Analysis of Pesticides and Veterinary Drug Residues in <i>Brassica rapa chinensis</i> Using an Improved Quechers Method Based on Magnetic Materials.

Foods (Basel, Switzerland)·2025
Same author

High sensitivity and specificity analysis of multiple miRNAs.

Analytica chimica acta·2025
Same author

Rapid detection of clenbuterol and ractopamine in pork: Sulfonate functionalized ion exchangemagnetic solid phase extraction combined with visual protein microarray chip.

Talanta·2025
Same author

A 3D-printed multi-channel microfluidic device for precise dissolved oxygen regulation in cancer hypoxia research.

Talanta·2025
Same author

Novel Sensing Strategy for MicroRNA via DSN-RCA Dual Amplification and Optical Tweezer-Assisted Suspension Bead Arrays.

Analytical chemistry·2025

Related Experiment Video

Updated: Mar 10, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

25.5K

Selection of aptamers based on a protein microarray integrated with a microfluidic chip.

Xiaohui Liu1, Hui Li1, Wenchao Jia1

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, China. lihui@nju.edu.cn xudanke@nju.edu.cn.

Lab on a Chip
|December 8, 2016
PubMed
Summary

We developed an efficient protein microarray integrated microfluidic chip for SELEX (systematic evolution of ligands by exponential enrichment) to screen aptamers. This PMM-SELEX method successfully identified specific aptamers for lactoferrin detection.

More Related Videos

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.7K
Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

6.9K

Related Experiment Videos

Last Updated: Mar 10, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

25.5K
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.7K
Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

6.9K

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Systematic evolution of ligands by exponential enrichment (SELEX) is crucial for aptamer discovery.
  • Traditional SELEX methods can be time-consuming and labor-intensive.
  • Developing efficient and rapid aptamer screening platforms is essential for molecular diagnostics.

Purpose of the Study:

  • To develop and validate an efficient and fast protein microarray integrated microfluidic chip for SELEX (PMM-SELEX).
  • To screen and identify high-affinity and specific aptamers targeting bovine lactoferrin.
  • To evaluate the performance of the selected aptamers in detecting lactoferrin.

Main Methods:

  • Preparation of a protein microarray with immobilized target (lactoferrin) and negative proteins (BSA, α-lactalbumin, β-lactoglobulin, casein).
  • Integration of the protein microarray into a microfluidic chip for the PMM-SELEX process over seven rounds.
  • Monitoring aptamer-target interactions using a fluorescence microarray scanner and validating aptamer affinity using Surface Plasmon Resonance (SPR).

Main Results:

  • Successfully obtained five specific and high-affinity aptamers (Lac-14, Lac-6a, Lac-9, Lac-5, Lac-3a) for lactoferrin after repeated selections.
  • Calculated dissociation constants (Kd) using SPR, confirming aptamer affinity.
  • Demonstrated the utility of aptamer Lac-6a for lactoferrin detection via fluorescence polarization with a linear response from 0.78-50 μg mL-1 and a detection limit of 0.39 μg mL-1.

Conclusions:

  • The PMM-SELEX method is a stable, accurate, and highly efficient platform for aptamer screening.
  • This innovative approach significantly enhances the speed and effectiveness of aptamer selection.
  • The selected aptamers show promise for sensitive and specific detection of lactoferrin in biological samples.