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ROS-INDUCED OXIDATIVE STRESS IN NOBILE-TYPE DENDROBIUM PROTOCORM-LIKE BODIES (PLBS) DURING VITRIFICATION
1Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China.
Background:
Oxidative stress involved in cryopreservation protocols may be responsible for the poor survival of tissues after cryopreservation.
Objective:
In the current study, we aimed to clarify the role of oxidative stress and its relationship with survival rate during cryopreservation of PLBs from nobile-type Dendrobium.
Materials And Methods:
ROS, antioxidants and oxidative products and the survival rate in PLBs from Dendrobium Hamanal Lake Dream were determined during vitrification.
Results:
Relative survival of PLBs decreased significantly after preculture and rewarming (P<0.01). Generation of ·O2- and protein carbonyl (PCO) increased significantly after preculture. Dramatic increases in ·O2-, H2O2 and MDA, and significant decreases in AsA content, activities of SOD and CAT were observed after rewarming.
Conclusion:
ROS-induced oxidative stress was associated with the poor survival of PLBs following vitrification. ·O2- was the predominant ROS resulting in the decreased survival after preculture, while H2O2 together with ·O2- appear to be responsible for the survival decrease after rewarming.
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