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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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Long Noncoding RNA Expression Signatures of Abdominal Aortic Aneurysm Revealed by Microarray
Yao Guo Yang1, Mei Xia Li2, Lei Kou1
1The Department of Vascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
Biomedical and Environmental Sciences : BES
|December 9, 2016
Summary
This study identified novel long noncoding RNA (lncRNA) candidates involved in abdominal aortic aneurysm (AAA) pathogenesis. One specific lncRNA, lnc-ARG, was found to decrease 5-lipoxygenase (ALOX5) expression and reactive oxygen species production.
Area of Science:
- Molecular Biology
- Genomics
- Cardiovascular Research
Background:
- Abdominal aortic aneurysm (AAA) is a life-threatening condition with complex pathogenesis.
- Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in various diseases, including cardiovascular conditions.
Purpose of the Study:
- To investigate the role of lncRNAs in the pathogenesis of abdominal aortic aneurysm (AAA).
- To identify novel lncRNA candidates associated with AAA development.
Main Methods:
- Microarray analysis of lncRNA and mRNA expression in AAA and normal aortic tissues.
- Real-time quantitative reverse-transcription PCR (qRT-PCR) for expression verification.
- Computational analysis to predict lncRNA-mRNA targeting relationships and protein-coding potential.
- In vitro experiments in HeLa cells to assess the effect of lnc-ARG on ALOX5 expression and reactive oxygen species (ROS) production.
Main Results:
- Identification of 3,688 differentially expressed lncRNAs and 3,007 differentially expressed mRNAs between AAA and normal tissues.
- Discovery of 1,284 differentially expressed long intergenic noncoding RNAs and 206 differentially expressed enhancer-like lncRNAs.
- Construction of a co-expression network linking differentially expressed lncRNAs and mRNAs.
- Demonstration that lnc-ARG, located upstream of ALOX5, reduces ALOX5 mRNA levels and ROS production in HeLa cells.
Conclusions:
- This study highlights the significant involvement of lncRNAs in AAA pathogenesis.
- Novel lncRNA candidates, including lnc-ARG, have been identified as potentially crucial players in AAA development and progression.
