Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.3K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.3K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

804
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
804
Proteomics01:33

Proteomics

10.0K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.0K
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

641
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
641

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pilot metaproteomic profiling reveals bacterial diversity and potential medical and veterinary relevance of tick microbiomes in northern Algeria.

Scientific reports·2026
Same author

Soluble CD95L triggers Caspase-10-driven reactive oxygen species production in neutrophils and aggravates anti-neutrophil cytoplasmic antibody-vasculitis.

Nature communications·2026
Same author

Specific and broad-spectrum antibacterial effectors of type VI secretion system drive competition of <i>Stenotrophomonas rhizophila</i> against bacteria from seed microbiota.

Microbiology spectrum·2026
Same author

ERO1a fosters glioblastoma aggressiveness and metabolic flexibility by regulating mitochondria-associated membrane dynamics.

Nature cell biology·2026
Same author

Pulsed light causes structural damage and proteomic alterations in Aspergillus brasiliensis conidia.

Food microbiology·2026
Same author

Structural characterization of an S8 protease from Fervidobacterium pennivorans reveals a unique tyrosine-rich β-hairpin.

Enzyme and microbial technology·2026

Related Experiment Video

Updated: Mar 10, 2026

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
08:31

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Published on: October 15, 2013

30.5K

Striking against bioterrorism with advanced proteomics and reference methods.

Jean Armengaud1

  • 1CEA, DRF, IBiTec-S, SPI, Li2D, Laboratory "Innovative technologies for Detection and Diagnostics", Bagnols-sur-Cèze, France.

Proteomics
|December 9, 2016
PubMed
Summary

A new multiplex assay using mass spectrometry (MS/MS) can accurately quantify eight dangerous toxins simultaneously. This method enhances bioweapon detection capabilities for national authorities, improving public safety against bioterrorism threats.

Keywords:
BioterrorismReference methodsSelected reaction monitoringStandardsTechnologyToxins

More Related Videos

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
05:37

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

Published on: October 20, 2020

7.5K
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

6.2K

Related Experiment Videos

Last Updated: Mar 10, 2026

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
08:31

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Published on: October 15, 2013

30.5K
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
05:37

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

Published on: October 20, 2020

7.5K
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

6.2K

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biosecurity

Background:

  • Biological toxins, such as ricin, are a significant concern as potential weapons.
  • Existing analytical methods struggle to detect multiple toxins simultaneously in complex matrices.
  • Enhanced detection capabilities are crucial for national authorities to monitor bioweapon threats.

Purpose of the Study:

  • To develop and validate a robust multiplex assay for the simultaneous quantification of multiple toxins.
  • To improve the analytical toolbox for detecting potential bioweapons.
  • To provide a reliable reference method for toxin analysis.

Main Methods:

  • Utilized mass spectrometry/mass spectrometry (MS/MS) for absolute quantitation.
  • Employed scheduled Selected Reaction Monitoring (SRM) transitions.
  • Developed high-quality, isotopically labeled toxin standards for accurate quantification.
  • Tested the assay in a complex food matrix.

Main Results:

  • Successfully quantified a set of eight toxins with good sensitivity, below their oral median lethal doses.
  • Demonstrated robust reliability of the MS/MS method, even with flexible SRM scheduling.
  • The assay showed advantageous sensitivity, discriminative power, and speed for multiplex toxin detection.

Conclusions:

  • The developed MS/MS reference assay provides a reliable and robust method for simultaneous toxin quantification.
  • Advanced proteomics techniques coupled with high-quality standards offer effective solutions for bioweapon monitoring.
  • This advancement strengthens national authorities' ability to respond to bioterrorism threats.