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Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

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Multi-dimensional super-resolution imaging enables surface hydrophobicity mapping.

Marie N Bongiovanni¹, Julien Godet², Mathew H Horrocks¹

¹Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.

Nature Communications

|December 9, 2016

Summary

This summary is machine-generated.

This study introduces a new super-resolution microscopy technique to map molecular hydrophobicity. The method simultaneously captures position and spectral data, revealing nanoscale environmental properties in biological systems.

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Area of Science:

Biophysics
Chemical Imaging
Nanotechnology

Background:

Super-resolution microscopy offers nanoscale insights into biological systems.
Current methods are limited to providing only positional information.
There is a need to extract richer environmental data at the nanoscale.

Purpose of the Study:

To develop a multi-dimensional super-resolution imaging technique.
To simultaneously determine the position and environmental properties of single-molecule fluorescent emitters.
To map the hydrophobicity of biological structures at the nanoscale.

Main Methods:

Exploiting the solvatochromic and fluorogenic properties of nile red dye.
Simultaneously extracting emission spectrum and position of each dye molecule.
Integrating a transmission diffraction grating into a localization-based super-resolution microscope.

Main Results:

Successfully mapped the hydrophobicity of synthetic lipid vesicles.
Visualized nanoscale hydrophobic changes in amyloid aggregates associated with neurodegenerative diseases.
Revealed hydrophobic alterations in mammalian cell membranes.

Conclusions:

The developed technique enables multi-dimensional super-resolution imaging.
This method provides simultaneous positional and environmental information from single emitters.
The technique is readily implementable and applicable to various biological systems.