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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

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Published on: April 7, 2014

Smartphone based hand-held quantitative phase microscope using the transport of intensity equation method.

Xin Meng¹, Huachuan Huang², Keding Yan³

¹Department of Optoelectronic Information Science and Engineering, School of Science, Jiangnan University, Wuxi, Jiangsu 214122, China. wsy_photonics@yahoo.com cheng.liu@hotmail.co.uk.

|December 9, 2016

Summary

A new hand-held smartphone microscope offers high-contrast imaging for mobile healthcare. This portable quantitative phase microscope uses a smartphone app for phase retrieval, enabling field-based medical diagnosis.

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Area of Science:

Biomedical Optics
Microscopy
Mobile Health

Background:

High-contrast imaging is crucial for medical diagnosis.
Portable and cost-effective microscopy solutions are needed for remote healthcare applications.

Purpose of the Study:

To demonstrate a hand-held smartphone-based quantitative phase microscope for mobile healthcare.
To develop a portable system for accurate, high-contrast imaging using readily available technology.

Main Methods:

Utilized a transport of intensity equation (TIE) method for phase retrieval.
Captured multi-focal images using a smartphone camera and manual focusing.
Developed an Android application to solve the Poisson equation for phase calculation.

Main Results:

Successfully imaged a random phase plate with known phases.
Demonstrated performance by imaging biological samples including red blood cells, Pap smears, and plant tissues.
Achieved accurate, high-contrast phase imaging with the portable system.

Conclusions:

The smartphone-based quantitative phase microscope is accurate, high-contrast, cost-effective, and field-portable.
This device shows promise as a tool for remote healthcare and medical diagnosis.
The developed system facilitates mobile healthcare by enabling advanced imaging in diverse settings.