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[Isoelectric focusing and electroblotting of human vitreous body proteins]
Due to its high resolution and reproducibility, isoelectric focusing with a highly sensitive silver stain, in combination with electroblotting and immunodetection, may be a valuable technique to provide detailed mapping of the protein pattern of the human vitreous. By means of this technique, the presence of albumin, alpha 1-antitrypsin, alpha 1-acid glycoprotein, and transferrin in the normal human vitreous was established. Our results indicate that the differences between vitreous protein und serum protein are more of a quantitative than a qualitative nature. Preliminary studies on pathological vitreous samples obtained during vitrectomy indicate that a substantial amount of the intravitreal protein in PVR as well as in proliferative diabetic retinopathy is plasma-derived. The vitreous from eyes with a macular pucker shows a pattern of protein bands that is reminiscent of normal vitreous.
Due to its high resolution and reproducibility, isoelectric focusing with a highly sensitive silver stain, in combination with electroblotting and immunodetection, may be a valuable technique to provide detailed mapping of the protein pattern of the human vitreous. By means of this technique, the presence of albumin, alpha 1-antitrypsin, alpha 1-acid glycoprotein, and transferrin in the normal human vitreous was established. Our results indicate that the differences between vitreous protein und serum protein are more of a quantitative than a qualitative nature. Preliminary studies on pathological vitreous samples obtained during vitrectomy indicate that a substantial amount of the intravitreal protein in PVR as well as in proliferative diabetic retinopathy is plasma-derived. The vitreous from eyes with a macular pucker shows a pattern of protein bands that is reminiscent of normal vitreous.