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Related Experiment Video

Updated: Mar 10, 2026

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
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Microfluidic immunosensor for rapid and highly-sensitive salivary cortisol quantification.

V Pinto1, P Sousa1, S O Catarino1

  • 1Microelectromechanical Systems Research Unit (CMEMS-UMinho), University of Minho, Campus de Azurém, 4800-058 Guimarães, Portugal.

Biosensors & Bioelectronics
|December 9, 2016
PubMed
Summary
This summary is machine-generated.

A novel poly(dimethylsiloxane) (PDMS) microfluidic immunosensor with CMOS optical detection enables rapid, sensitive salivary cortisol quantification. This non-invasive method offers a promising alternative for continuous point-of-care monitoring.

Keywords:
Colorimetric detectionLab-on-a-chip (LOC)Microfluidic immunosensorPoly(dimethylsiloxane)Salivary cortisol

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Materials Science

Background:

  • Salivary cortisol measurement is crucial for diagnosing stress-related disorders and monitoring circadian rhythms.
  • Traditional methods for cortisol analysis often require invasive blood sampling and complex laboratory procedures.
  • There is a need for rapid, sensitive, and non-invasive point-of-care diagnostic tools for salivary cortisol.

Purpose of the Study:

  • To develop and validate a novel poly(dimethylsiloxane) (PDMS) microfluidic immunosensor integrated with a complementary metal-oxide-semiconductor (CMOS) optical detection system.
  • To achieve rapid and highly-sensitive quantification of salivary cortisol using a non-invasive saliva sample.
  • To explore the potential of this device for continuous monitoring in point-of-care applications.

Main Methods:

  • Fabrication of a PDMS microfluidic chip with covalent immobilization of a coating antibody (Ab) and a capture antibody specific for cortisol.
  • Development of a competitive immunoassay using horseradish peroxidase (HRP)-labelled cortisol.
  • Colorimetric detection of the HRP-tetramethylbenzidine (TMB) reaction product via optical absorption at 450nm using a CMOS silicon photodiode.

Main Results:

  • The developed immunosensor demonstrated a linear detection range of 0.01-20 ng/mL for salivary cortisol.
  • Achieved a highly sensitive limit of detection (LOD) of 18 pg/mL.
  • The entire analysis time for cortisol quantification was optimized to 35 minutes.

Conclusions:

  • The novel PDMS microfluidic immunosensor with CMOS detection provides a rapid, sensitive, and non-invasive method for salivary cortisol quantification.
  • The device shows significant potential for point-of-care applications, enabling continuous monitoring of cortisol levels, such as during a circadian cycle.
  • This technology offers a valuable alternative to traditional blood-based cortisol analysis for clinical diagnostics.