Generation and characterization of an induced pluripotent stem cell (iPSC) line from a patient with
Fabio Marsoner1, Matteo Marcatili2, Thodoris Karnavas3
1Laboratory of Stem Cell Biology, Centre for Integrative Biology - CIBIO, Università degli Studi di Trento, Trento, Italy.
Abstract:
Peripheral Blood Mononuclear Cells (PBMCs) were collected from a patient with clozapine-resistant (also known as "super-refractory") Schizophrenia. iPSCs were established with a non-integrating Sendai virus-based reprogramming system. A footprint-free hiPSC line was characterized to express the main endogenous pluripotency markers and to retain a normal karyotype. Cells showed pluripotency competency by giving rise to progeny of differentiated cells belonging to the three germ layers. This hiPSC line represents a valuable tool to obtain mature, pathology-relevant neuronal populations in vitro that are suitable to investigate the molecular background of the schizophrenic disorder and the resultant patients' response to treatments.
More Related Videos
09:30A Guide to Generating and Using hiPSC Derived NPCs for the Study of Neurological Diseases
Published on: February 21, 2015
10:48Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions
Published on: November 26, 2014
Related Concept Videos
Induced Pluripotent Stem Cells
Induced Pluripotent Stem Cells
Somatic...
EPS and iPS Cells in Disease Research
iPS Cell Differentiation
