Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Flora of the Large Intestine01:29

Bacterial Flora of the Large Intestine

1.8K
The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
1.8K
Bacterial Phylum Verrucomicrobiota01:26

Bacterial Phylum Verrucomicrobiota

654
The phylum Verrucomicrobiota comprises at least four characterized orders, with most species classified within the order Verrucomicrobiotales. Members of this phylum are either aerobic or facultatively aerobic, with the ability to ferment sugars. A notable exception is the genus Methylacidiphilum, which consists of aerobic methanotrophs. Additionally, some Verrucomicrobiota establish symbiotic relationships with protists. These bacteria are widely distributed across various environments,...
654
Bacterial Phylum Firmicutes01:27

Bacterial Phylum Firmicutes

1.2K
Firmicutes is a diverse phylum of Gram-positive bacteria characterized by a low GC content in their genomes. This phylum includes organisms with monoderm or diderm cell envelopes, highlighting a complex evolutionary history. Firmicutes comprises several major orders, including Lactobacillales, Clostridiales, and Bacillales, which exhibit remarkable diversity in their morphology, metabolism, and ecological roles.The order Lactobacillales includes lactic acid bacteria, which are fermentative...
1.2K
Diversity of Archaea IV01:29

Diversity of Archaea IV

570
Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
570
Other Unique Bacteria01:18

Other Unique Bacteria

519
Magnetic bacteria exhibit a directed movement called magnetotaxis, driven by structures called magnetosomes. These magnetosomes consist of chains of magnetic particles made of either magnetite (Fe₃O₄) or greigite (Fe₃S₄) and are organized in a linear conformation by a protein scaffold within invaginations of the cell membrane. The bacteria align along the north–south magnetic field lines, much like a compass needle. They are typically microaerophilic or anaerobic...
519
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

1.3K
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pimozide Inhibits Type II but Not Type I Hair Cells in Chicken Embryo and Adult Mouse Vestibular Organs.

Biomedicines·2025
Same author

Metatranscriptome and Resistome of the Endodontic Microbiome.

Journal of endodontics·2024
Same author

Corrigendum: Precision medicine: a new era for inner ear diseases.

Frontiers in pharmacology·2024
Same author

A hybrid receptor binding protein enables phage F341 infection of <i>Campylobacter</i> by binding to flagella and lipooligosaccharides.

Frontiers in microbiology·2024
Same author

Precision medicine: a new era for inner ear diseases.

Frontiers in pharmacology·2024
Same author

Epigenetics and transgenerational inheritance.

The Journal of physiology·2023

Related Experiment Video

Updated: Mar 10, 2026

Preparation and Maintenance of Bioexclusion IsoPositive Cage Experiment for Human Fecal Transplantation into Germ-Free Mice
07:45

Preparation and Maintenance of Bioexclusion IsoPositive Cage Experiment for Human Fecal Transplantation into Germ-Free Mice

Published on: February 28, 2025

1.3K

Stable core virome despite variable microbiome after fecal transfer.

Felix Broecker1,2,3, Giancarlo Russo4, Jochen Klumpp5

  • 1a Institute of Medical Microbiology , University of Zurich , Zurich , Switzerland.

Gut Microbes
|December 10, 2016
PubMed
Summary

Fecal microbiota transplantation (FMT) led to a stable, donor-like phage population in a patient with recurrent Clostridium difficile infection (rCDI). This stable virome correlated with successful FMT, unlike the more variable bacterial microbiota.

Keywords:
Clostridium difficilecore viromefecal microbiota transplantationlong-term analysismicrobiomesequencingvirome

More Related Videos

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

20.8K
Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
06:23

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

Published on: February 15, 2019

15.0K

Related Experiment Videos

Last Updated: Mar 10, 2026

Preparation and Maintenance of Bioexclusion IsoPositive Cage Experiment for Human Fecal Transplantation into Germ-Free Mice
07:45

Preparation and Maintenance of Bioexclusion IsoPositive Cage Experiment for Human Fecal Transplantation into Germ-Free Mice

Published on: February 28, 2025

1.3K
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

20.8K
Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
06:23

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

Published on: February 15, 2019

15.0K

Area of Science:

  • Microbiology
  • Virology
  • Gastroenterology

Background:

  • Recurrent Clostridium difficile infection (rCDI) poses a significant clinical challenge.
  • Fecal microbiota transplantation (FMT) is an effective treatment for rCDI, modulating the gut microbiome.
  • The enteric virome's role in FMT success and gut health remains incompletely understood.

Purpose of the Study:

  • To investigate the long-term dynamics of the enteric virome post-FMT in an rCDI patient.
  • To compare the stability and characteristics of the viral and bacterial communities after FMT.
  • To explore the correlation between virome stability and successful FMT outcomes.

Main Methods:

  • Longitudinal analysis of enteric virome and bacterial microbiota over 4.5 years.
  • Next-generation sequencing for viral and bacterial community profiling.
  • Bioinformatic analysis of viral populations, including diversity, richness, and donor similarity.

Main Results:

  • The patient's phage population rapidly acquired donor-similar characteristics post-FMT, remaining stable for up to 7 months.
  • Viral diversity and richness in the patient mirrored those of the donor.
  • In contrast, the bacterial microbiota showed significant variability and dysbiosis for over 7 months before achieving donor similarity.

Conclusions:

  • Stable phage populations, potentially representing a core virome, may be a better indicator of successful FMT than dynamic bacterial communities.
  • The enteric virome's stability post-FMT warrants further investigation as a predictor of clinical outcomes.
  • Understanding the virome's role could inform FMT strategies for rCDI and other gut-related diseases.