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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

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Quantitative Detection of Trace Explosive Vapors by Programmed Temperature Desorption Gas Chromatography-Electron Capture Detector

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Calibrant-Free Analyte Quantitation via a Variable Velocity Flow Cell.

Jason G Beck^1,2, Aleksander Skuratovsky^3,2, Michael C Granger^3,2,4

¹Department of Chemistry, University of Utah , Salt Lake City, Utah 84112, United States.

Analytical Chemistry

|December 10, 2016

Summary

This study introduces a new analyte quantitation method using fluid dynamics and sensor signals, eliminating the need for traditional calibration curves. This approach offers rapid and accurate concentration measurements for analytes like ferrocenedimethanol (FDM).

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Area of Science:

Analytical Chemistry
Electrochemistry
Fluid Dynamics

Background:

Traditional analyte quantitation relies on calibrants, internal standards, and calibration curves, which can be time-consuming and resource-intensive.
Developing novel analytical methods that circumvent these requirements is crucial for efficient chemical analysis.

Purpose of the Study:

To present a novel method for analyte quantitation that does not require calibrants, internal standards, or calibration curves.
To demonstrate the feasibility of using surface-directed analyte flux and signal response for accurate concentration determination.

Main Methods:

Fabrication of two flow cells with an array of electrodes along the flow axis.
Systematic variation of mean linear fluid velocity (U) to control analyte flux.
Measurement of resultant limiting currents for a redox analyte (ferrocenedimethanol, FDM).
Application of a convective-diffusive transport model for concentration calculation.

Main Results:

Demonstrated predictable variation of address-directed analyte flux with fluid velocity.
Accurate description of measured limiting currents using a convective-diffusive transport model.
Achieved concentration calculations with less than 0.5% deviation from true values using empirically derived U.
Completed experiment and concentration calculation in minutes.

Conclusions:

The novel method successfully quantitates analyte concentrations without traditional calibration techniques.
The approach leverages predictable relationships between fluid dynamics, analyte transport, and sensor signals.
This method offers a rapid, accurate, and potentially broadly applicable alternative for chemical analysis.