Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Redox Reactions01:27

Redox Reactions

1.3K
Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
1.3K
Redox Reactions01:24

Redox Reactions

59.2K
Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
59.2K
Oxygen Requirements and Growth Patterns01:29

Oxygen Requirements and Growth Patterns

2.0K
Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
2.0K
Radical Autoxidation01:20

Radical Autoxidation

3.3K
The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
3.3K
Oxidation and Reduction of Organic Molecules01:19

Oxidation and Reduction of Organic Molecules

9.8K
Energy production within a cell involves many coordinated chemical pathways. Most of these pathways are combinations of oxidation and reduction reactions, which occur at the same time. An oxidation reaction strips an electron from an atom in a compound, and the addition of this electron to another compound is a reduction reaction. Because oxidation and reduction usually occur together, these pairs of reactions are called redox reactions.
The removal of an electron from a molecule, results in a...
9.8K
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

9.5K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inceptor binds to and directs insulin towards lysosomal degradation in β cells.

Nature metabolism·2024
Same author

Patterned thin film enzyme electrodes <i>via</i> spincoating and glutaraldehyde vapor crosslinking: towards scalable fabrication of integrated sensor-on-CMOS devices.

Lab on a chip·2024
Same author

The Myxobacterial Antibiotic Myxovalargin: Biosynthesis, Structural Revision, Total Synthesis, and Molecular Characterization of Ribosomal Inhibition.

Journal of the American Chemical Society·2023
Same author

Phenotypic drug screening in a human fibrosis model identified a novel class of antifibrotic therapeutics.

Science advances·2021
Same author

TRAF6 prevents fatal inflammation by homeostatic suppression of MALT1 protease.

Science immunology·2021
Same author

Computer-Aided Design and Synthesis of a New Class of PEX14 Inhibitors: Substituted 2,3,4,5-Tetrahydrobenzo[F][1,4]oxazepines as Potential New Trypanocidal Agents.

Journal of chemical information and modeling·2021

Related Experiment Video

Updated: Mar 10, 2026

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
10:05

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Published on: May 8, 2020

2.3K

What Do Reactive Fragments Actually Do in Cells?

Oliver Plettenburg1,2

  • 1Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt GmbH, Institute of Medicinal Chemistry, Ingolstädter Landstrasse 1, 85764, Neuherberg, Germany.

Angewandte Chemie (International Ed. in English)
|December 10, 2016
PubMed
Summary

Reactive fragments, often considered promiscuous, were studied for their binding selectivities. This research examined their cellular target proteins across the entire proteome.

Keywords:
covalent fragmentsproteomicstarget identification

More Related Videos

Production and Detection of Reactive Oxygen Species ROS in Cancers
07:17

Production and Detection of Reactive Oxygen Species ROS in Cancers

Published on: November 21, 2011

71.2K
Author Spotlight: High-Throughput Measurement of Intracellular ROS Levels in Hepatocellular Lines
05:16

Author Spotlight: High-Throughput Measurement of Intracellular ROS Levels in Hepatocellular Lines

Published on: January 19, 2024

6.9K

Related Experiment Videos

Last Updated: Mar 10, 2026

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
10:05

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Published on: May 8, 2020

2.3K
Production and Detection of Reactive Oxygen Species ROS in Cancers
07:17

Production and Detection of Reactive Oxygen Species ROS in Cancers

Published on: November 21, 2011

71.2K
Author Spotlight: High-Throughput Measurement of Intracellular ROS Levels in Hepatocellular Lines
05:16

Author Spotlight: High-Throughput Measurement of Intracellular ROS Levels in Hepatocellular Lines

Published on: January 19, 2024

6.9K

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Proteomics

Background:

  • Covalently binding molecules are often assumed to exhibit broad, non-specific interactions.
  • Understanding the specificity of reactive molecules is crucial for drug discovery and chemical biology.
  • The cellular targets of many reactive fragments remain incompletely characterized.

Purpose of the Study:

  • To investigate the binding selectivity of diverse reactive fragments.
  • To identify the specific cellular target proteins of these fragments within a proteome-wide context.
  • To challenge the assumption of general promiscuity for covalently binding molecules.

Main Methods:

  • Utilized a broad range of reactive fragments for testing.
  • Employed proteome-wide screening techniques to identify binding interactions.
  • Analyzed binding data to determine selectivity profiles and specific protein targets.

Main Results:

  • Demonstrated that many reactive fragments exhibit specific binding patterns, contrary to general assumptions.
  • Identified a defined set of cellular target proteins for various reactive fragments.
  • Quantified the binding selectivity across the proteome for each fragment tested.

Conclusions:

  • The promiscuity of covalently binding molecules is not universal; specificities exist.
  • This study provides a valuable resource for understanding reactive fragment interactions.
  • Findings have implications for the rational design of targeted covalent inhibitors and chemical probes.