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The lack of long-term suppressive effect of ciprofloxacin on murine bone marrow
1Unit of Infectious Diseases, Chaim Sheba Medical Center, Tel-Hashomer, Israel.
Abstract:
In order to investigate the long-term effect of therapy with ciprofloxacin on haemopoietic cells, we administered ciprofloxacin orally in a daily dosage of 150 mg/kg/day in three divided doses for 11 days to syngeneic mice. A control group of mice was treated with saline. On the second, sixth, tenth and sixteenth day of ciprofloxacin therapy complete blood counts (cbc) and determination of haemopoietic progenitor cells (cfu-gm) from the tibial and femoral bone marrow were performed and followed by the transplantation of the bone marrow into lethally irradiated syngeneic mice. Bone marrow engraftment was evaluated ten days following transplantation by counting the visible colonies formed on the spleen surface (cfu-s) and by measuring the incorporation of 125I by the spleen and by the femurs of the transplanted mice. The results revealed that there was no statistical significance between the control and the ciprofloxacin treated mice in cbc, cfu-gm and cfu-s parameters. It is concluded that the suppressive effect of ciprofloxacin on haemopoietic cells appears only with very high concentrations of ciprofloxacin and is short-term and reversible.
Insights
Ciprofloxacin therapy did not significantly impact blood cell counts or bone marrow stem cells in mice. The antibiotic
Area of Science:
- Pharmacology
- Hematology
- Toxicology
Background:
- Ciprofloxacin is a widely used antibiotic.
- Its potential long-term effects on the hematopoietic system require investigation.
Purpose of the Study:
- To evaluate the long-term effects of ciprofloxacin on mouse hematopoietic cells.
- To determine if ciprofloxacin affects complete blood counts (CBC) and hematopoietic progenitor cells (CFU-GM).
Main Methods:
- Syngeneic mice received daily oral ciprofloxacin (150 mg/kg/day) for 11 days; controls received saline.
- Complete blood counts (CBC) and bone marrow progenitor cells (CFU-GM) were assessed on days 2, 6, 10, and 16.
- Bone marrow was transplanted into irradiated mice to evaluate spleen colony-forming units (CFU-S) and radiotracer incorporation.
Main Results:
- No statistically significant differences were observed between ciprofloxacin-treated and control groups in CBC, CFU-GM, or CFU-S.
- Hematopoietic parameters remained unaffected by the ciprofloxacin dosage used in the study.
Conclusions:
- Ciprofloxacin, at the tested dosage, does not cause long-term suppression of hematopoietic cells in mice.
- Significant suppressive effects on hematopoietic cells likely require much higher ciprofloxacin concentrations and are reversible.