Photoswitchable Emission Color Change in Nanodots Containing Conjugated Polymer and Photochrome

A simple approach for the preparation of conjugated polymer (CP)-based fluorescent nanodots containing photochrome (dithienylethene, DTE) is reported. The CP in the nanodots was designed to exhibit dual emissions of blue and green. The photochromic, fluorescent, composite nanodots (PNDs) were able to tune the emission color from green to blue using selective energy transfer from the CP to DTE under ultraviolet (UV) irradiation. The UV-irradiation-induced ring closure of the DTE within the PNDs provided a spectral overlap between the green emission of the CP and the absorption of DTE, leading to quenching of the green emission and, concomitantly, maintaining of the blue emission. The photoswitchable fluorescent PNDs with high on-off green fluorescence contrast were successfully applied in a living zebrafish imaging. Our design strategy provided a versatile tool for constructing a special photomodulated color-changeable nanostructure in bioimaging.