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Related Experiment Videos

Do transvascular forces in isolated lobe preparations equilibrate?

B J Hancock1, M Hoppensack, L Oppenheimer

  • 1Department of Surgery, University of Manitoba, Winnipeg, Canada.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|August 1, 1989
PubMed
Summary

This study reveals that canine lung microvasculature has low filtration conductance (Kf) and high albumin reflection coefficient (sigma d), indicating a well-preserved state. The critical pressure (Pcrit) for filtration was lower than previously reported.

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Area of Science:

  • Pulmonary Physiology
  • Cardiovascular Research
  • Microvascular Dynamics

Background:

  • Understanding microvascular pressure-filtration relationships is crucial for lung fluid balance.
  • Previous studies often used different experimental conditions, potentially affecting critical pressure (Pcrit) interpretations.

Purpose of the Study:

  • To investigate the relationship between constant weight gain rate (Qf) and microvascular pressure (Pc) in isolated canine left lower lobes.
  • To determine lobar filtration conductance (Kf) and critical pressure (Pcrit).
  • To estimate the microvascular reflection coefficient for albumin (sigma d).

Main Methods:

  • Stepwise pressure elevation technique to measure Qf and Pc.
  • Perfusate colloid osmotic pressure (II) manipulation using albumin.

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  • Analysis of weight changes over time under varying pressures.

    • Main Results:

    • Lobar filtration conductance (Kf) was 0.0022 +/- 0.003 ml.min-1.cmH2O-1.g dry wt-1.
    • Critical pressure (Pcrit) was 9.53 +/- 1.18 cmH2O, lower than typically used.
    • Increasing perfusate colloid osmotic pressure with albumin significantly increased Pcrit and yielded a sigma d of 0.67.

    Conclusions:

    • The canine lung microvasculature exhibits low filtration and high albumin reflection, suggesting good preservation.
    • The determined low Pcrit may indicate lymphatic channel obliteration.
    • The weight transient method for measuring Kf is reliable within a specific pressure range.